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Abstract ▼ AI-Helper

The DgLsL anti-sense partial gene (526 bp) was introduced into chrysanthemum (Dendranthema grandiflorum Kitamura) ‘Zinba’ by co-cultivation with Agrobacterium tumefaciens strain C58C1 (pCAMBIA2300). After infection of leaf segments with A. tumafaciens, infected explants were cultured on a regenerati...

The DgLsL anti-sense partial gene (526 bp) was introduced into chrysanthemum (Dendranthema grandiflorum Kitamura) ‘Zinba’ by co-cultivation with Agrobacterium tumefaciens strain C58C1 (pCAMBIA2300). After infection of leaf segments with A. tumafaciens, infected explants were cultured on a regeneration medium ((RM: MS basal salts + 1.0 ㎎･L-1 BA + 0.5 ㎎･L-1 IAA + 10 ㎎･L-1 kanamycin, pH 5.8) with 0.7% plant agar (Duchefa, The Netherlands)) as the first selection medium, and then were transferred to the second selection medium containing 20 ㎎･L-1 kanamycin. After a selection process in the selection RM, putatively-transformed explants were placed on a rooting medium (MS basal salts + 20 ㎎･L-1 kanamycin) as the third selection. Until the third selection, 220 plantlets (3.6%) of total inoculated explants (6,740 explants) survived and rooted. Also, 101 plantlets (1.6%) among them were confirmed as putative transformants with the nptII gene by PCR analysis. Using Southern blot analysis, 86 (1.5%) out of 101 plantlets were confirmed as transformants with the nptII gene and the DgLsL anti-sense partial gene. The transformation efficiency of DgLsL anti-sense partial gene was 1.5%. All transformants with DgLsL anti-sense partial gene and non-transformants were acclimatized and established well in a greenhouse. Lateral branches were observed in both transformants and non-gene transformed chrysanthemums. However, three transformed lines (LS2, LS9, and LS10) with a DgLsL partial anti-sense gene showed early flowering.

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