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Abstract ▼ AI-Helper

The gametophytes of Asplenium incisum, Camptosorus sibiricus, and Phyllitis scolopendrium derived from spores were mechanically homogenized and cultured in vitro to study their gametophyte development and gametanginial ontogeny. The regeneration speed from homogenized gametophytes was faster in P. s...

The gametophytes of Asplenium incisum, Camptosorus sibiricus, and Phyllitis scolopendrium derived from spores were mechanically homogenized and cultured in vitro to study their gametophyte development and gametanginial ontogeny. The regeneration speed from homogenized gametophytes was faster in P. scolopendrium than A. incisum or C. sibiricus, so spatulate gamatophytes were most rapidly formed in P. scolopendrium after 2 weeks in culture. The gametangia formation was seen at week 6 to 8 in all three species. The female gametophytes first appeared and later, bisexual gamatophytes were observed. But development of sporophytes was not observed for 14 weeks in all three species. Unfertilized archegonia were eventually degenerated by surrounding with thick cell layers.

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