[논문]Genotypic Characterization of Escherichia coli O157:H7 Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis

Ateba, Collins Njie; Mbewe, Moses

doi:10.3390/ijms15069735

Genotypic Characterization of Escherichia coli O157:H7 Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis 원문보기

International journal of molecular sciences, v.15 no.6, 2014년, pp.9735 - 9747

Ateba, Collins Njie (Department of Biological Sciences, North West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa) , Mbewe, Moses (Department of Water and Sanitation, University of Limpopo, Turfloop Campus, Private Bag X1106, Sovenga 0727, South Africa)

Abstract ▼ AI-Helper

In many developing countries, proper hygiene is not strictly implemented when animals are slaughtered and meat products become contaminated. Contaminated meat may contain Escherichia coli (E.coli) O157:H7 that could cause diseases in humans if these food products are consumed undercooked. In the present study, a total of 94 confirmed E. coli O157:H7 isolates were subjected to the enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR) typing to generate genetic fingerprints. The ERIC fragments were resolved by electrophoresis on 2% (w/v) agarose gels. The presence, absence and intensity of band data were obtained, exported to Microsoft Excel (Microsoft Office 2003) and used to generate a data matrix. The unweighted pair group method with arithmetic mean (UPGMA) and complete linkage algorithms were used to analyze the percentage of similarity and matrix data. Relationships between the various profiles and/or lanes were expressed as dendrograms. Data from groups of related lanes were compiled and reported on cluster tables. ERIC fragments ranged from one to 15 per isolate, and their sizes varied from 0.25 to 0.771 kb. A large proportion of the isolates produced an ERIC banding pattern with three duplets ranging in sizes from 0.408 to 0.628 kb. Eight major clusters (I–VIII) were identified. Overall, the remarkable similarities (72% to 91%) between the ERIC profiles for the isolate from animal species and their corresponding food products indicated some form of contamination, which may not exclude those at the level of the abattoirs. These results reveal that ERIC PCR analysis can be reliable in comparing the genetic profiles of E.coli O157:H7 from different sources in the North-West Province of South Africa.

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참고문헌 (33)

1. Cowden J.M. Ahmed S. Donaghy M. Riley A. Epidemiological investigation of the central Scotland outbreak of Escherichia coli O157 infection, November to December 1996 Epidemiol. Infect. 2001 126 335 341 11467789
2. Dundas S. Todd W.T. Stewart A.I. Murdoch P.S. Chaudhuri A.K. Hutchinson S.J. The central Scotland Escherichia coli O157: H7 outbreak: Risk factors for the haemolytic uremic syndrome and death among hospitalized patients Clin. Infect. Dis. 2001 33 923 931 10.1086/322598 11528561

상세보기
3. Tozzi A.E. Caprioli A. Minelli F. Gianviti A. de Petris L. Edefonti A. Montini G. Ferretti A. de Palo T. Gaido M. Shiga toxin-producing Escherichia coli infections associated with haemolytic uremic syndrome, Italy, 1988–2000 Emerg. Infect. Dis. 2003 9 106 108 10.3201/eid0901.020266 12533290

상세보기
4. Beutin L. Krause G. Zimmermann S. Kaulfuss S. Gleier K. Characterisation of Shiga toxin-producing E. coli strains isolated from human patients in Germany over a 3-year period Clin. Microbiol. 2004 42 1099 1180 10.1128/JCM.42.3.1099-1108.2004

상세보기
5. Chang H.G. Tserenpuntsag B. Kacica M. Smith P.F. Morse D.L. Haemolytic uraemic syndrome incidence in New York Emerg. Infect. Dis. 2004 10 928 931 10.3201/eid1005.030456 15200834

상세보기
6. Lynn R.M. O’Brien S.J. Taylor C.M. Adak G.K. Chart H. Cheasty T. Coia J.E. Gillespie I.A. Locking M.E. Reilly W.J. Childhood haemolytic uraemic syndrome, United Kingdom and Ireland Emerg. Infect. Dis. 2005 11 590 596 10.3201/eid1104.040833 15829199

상세보기
7. Müller E.E. Ehlers M.M. Grabow W.O.K. The occurrence of E. coli O157:H7 in Southern African water sources intended for direct and indirect human consumption Water Res. 2001 35 3085 3088 10.1016/S0043-1354(00)00597-2 11487104

상세보기
8. Nataro J.P. Kaper J.B. Diarrhoeagenic Escherichia coli Clin. Microbiol. Rev. 1998 11 142 201 9457432
9. Olsen S.J. Miller G. Breuer T. Kennedy M. Higgins C. Walford J. McKee G. Fox K. Bibb W. Mead P. A waterborne outbreak of Escherichia coli O157 infections and haemolytic uraemic syndrome: Implications for rural water systems Emerg. Infect. Dis. 2002 8 370 375 10.3201/eid0804.000218 11971769

상세보기
10. Igarashi T. Inatomi J. Wake A. Takamizawa M. Katayama H. Iwata T. Failure of pre-diarrheal antibiotics to prevent haemolytic uraemic syndrome in serologically proven Escherichia coli O157:H7 gastrointestinal infection J. Paediat. 1999 135 768 769 10.1016/S0022-3476(99)70100-9

상세보기
11. Uhitil S. Jakšic S. Petrak T. Botka-Petrak K. Presence of Escherichia coli O157:H7 in ground beef and ground baby beef meat Food Protect. 2004 64 862 864
12. Law D. Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli. Appl. Microbiol. 2000 88 729 745 10.1046/j.1365-2672.2000.01031.x

상세보기
13. Paton A.W. Paton C.J. Direct detection and characterisation of Shiga toxigenic Escherichia coli by multiplex PCR for stx 1 , stx 2 , eae , ehxA and saa Clin. Microbiol. 2002 40 271 274 10.1128/JCM.40.1.271-274.2002

상세보기
14. Pierard D. Muyldermans G. Moriau L. Stevens D. Lauwers S. Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates Clin. Microbiol. 1998 36 3317 3322
15. Schmidt H. Scheef J. Morabito S. Caprioli A. Wieler L.H. Karch H. A new Shiga toxin 2 variant ( Stx2f ) from Escherichia coli isolated from pigeons Appl. Environ. Microbiol. 2000 66 1205 1208 10.1128/AEM.66.3.1205-1208.2000 10698793

상세보기
16. Jay M.T. Garrett V. Mohle-Boetani J.C. Barros M. Farrar J.A. Rios R. Abbott S. Sowadsky R. Komatsu K. Mandrell R. A multistate outbreak of Escherichia coli O157:H7 infection linked to consumption of beef tacos at a fast-food restaurant chain Clin. Infect. Dis. 2004 39 1 7 10.1086/421088 15206044

상세보기
17. Laine E.S. Scheftel J.M. Boxrud D.J. Vought K.J. Danila R.N. Elfering K.M. Outbreak of Escherichia coli O157:H7 infections associated with no intact blade-tenderized frozen steaks sold by door-to-door vendors Food Protect. 2005 68 1198 2002
18. Magwira C.A. Gashe B.A. Collison E.K. Prevalence and antibiotic resistance profiles of Escherichia coli O157:H7 in beef products from retail outlets in Gaborone, Botswana Food Protect. 2005 68 403 406
19. Maruzumi M. Morita M. Matsouka Y. Uekawa A. Nakamura T. Fugi K. Mass food poisoning caused by beef offal contaminated by Escherichia coli O157 Jpn. J. Infect. Dis. 2005 58 397 16377880
20. Chapman P.A. Siddons C.A. Wright D.J. Norman P. Fox J. Crick E. Cattle as a possible source of verocytotoxin-producing Escherichia coli O157 in man Epidemiol. Infect. 1993 111 439 447 10.1017/S0950268800057162 8270004

상세보기
21. Johnsen G. Wasteson Y. Heir E. Berget O.I. Herikstad H. Escherichia coli O157:H7 in faeces from cattle, sheep and pigs in the southwest part of Norway during 1998 and 1999 Int. J. Food Microbiol. 2001 65 193 200 10.1016/S0168-1605(00)00518-3 11393688

상세보기
22. Ateba C.N. Mbewe M. Bezuidenhout C.C. The prevalence of Escherichia coli O157 strains in cattle, pigs and humans in the North-West Province, South Africa SAJS 2008 104 7 8
23. Fegan N. Vanderlinde P. Higgs G. Desmarchelier P. The prevalence and concentration of Escherichia coli O157 in faeces of cattle from different production systems at slaughter Appl. Microbiol. 2004 97 362 370 10.1111/j.1365-2672.2004.02300.x

상세보기
24. Versalovic J. Schneider M. de Bruijn F.J. Lupski J.R. Genomic fingerprinting of bacteria with repetitive sequence-based polymerase chain reaction Methods Mol. Cell Biol. 1994 5 25 40
25. Mohapatra B.R. Broersma K. Mazumder A. Comparison of five Rep-PCR genomic fingerprinting methods for differentiation of faecal Escherichia coli from humans, poultry and wild birds FEMS Microbiol. Lett. 2007 277 98 106 10.1111/j.1574-6968.2007.00948.x 17986090

상세보기
26. Ateba C.N. Mbewe M. Detection of E. coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the North-West Province, South Africa: Public health implications Res. Microbiol. 2011 162 240 248 10.1016/j.resmic.2010.11.008 21272634

상세보기
27. Knutton S. Baldwin T. Williams H. McNeish A.S. Actin accumulation at sites of bacterial adhesion to tissue culture cells: Basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli Infect. Immun. 1989 57 1290 1298 2647635

상세보기
28. Omisakin F. Macrae M. Ogden I.D. Strachan N.J. Concentration and prevalence of Escherichia coli O157 in faeces at slaughter Appl. Environ. Microbiol. 2003 69 2444 2447 10.1128/AEM.69.5.2444-2447.2003 12732509

상세보기
29. Ateba C.N. Bezuidenhout C.C. Characterisation of Escherichia coli O157 strains from humans, cattle and pigs in the North-West Province, South Africa Int. J. Food Microbiol. 2008 128 181 188 10.1016/j.ijfoodmicro.2008.08.011 18848733

상세보기
30. Panangala V.S. van Santen V.L. Shoemaker C.A. Klesius P.H. Analysis of 16S–23S intergenic spacer regions of the rRNA operons in Edwardsiella ictaluri and Edwardsiella tarda isolates from fish Appl. Microbiol. 2005 99 657 669 10.1111/j.1365-2672.2005.02626.x

상세보기
31. Nielsen E.M. Scheutz F. Characterization of Escherichia coli O157 isolates from Danish cattle and human patients by genotyping and presence and variants of virulence genes Vet. Microbiol. 2002 88 259 273 10.1016/S0378-1135(02)00107-4 12151200

상세보기
32. Meichtri L. Miliwebsky E. Gioffré A. Chinen I. Baschkier A. Chillemi G. Guth B.E. Masana M.O. Cataldi A. Rodríguez H.R. Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: Prevalence and virulence properties Int. J. Food Microbiol. 2004 96 189 198 10.1016/j.ijfoodmicro.2004.03.018 15364473

상세보기
33. Sambrook J. Fritsch E.F. Maniatis T. Molecular Cloning, A Laboratory Manual 2nd ed. Cold Spring Harbor Laboratory Press New York, NY, USA 1989

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Genotypic Characterization of Escherichia coli O157:H7 Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis 원문보기

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