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An Alternative Method to Facilitate cDNA Cloning for Expression Studies in Mammalian Cells by Introducing Positive Blue White Selection in Vaccinia Topoisomerase I-Mediated Recombination

PloS one, v.10 no.9, 2015년, pp.e0139349 -   

Udo, Hiroshi (Department of Biology, Graduate School of Science, Kyushu University, Fukuoka, Japan)

Abstract AI-Helper 아이콘AI-Helper

One of the most basic techniques in biomedical research is cDNA cloning for expression studies in mammalian cells. Vaccinia topoisomerase I-mediated cloning (TOPO cloning by Invitrogen) allows fast and efficient recombination of PCR-amplified DNAs. Among TOPO vectors, a pcDNA3.1 directional cloning ...

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