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NTIS 바로가기PloS one, v.10 no.9, 2015년, pp.e0139349 -
Udo, Hiroshi (Department of Biology, Graduate School of Science, Kyushu University, Fukuoka, Japan)
One of the most basic techniques in biomedical research is cDNA cloning for expression studies in mammalian cells. Vaccinia topoisomerase I-mediated cloning (TOPO cloning by Invitrogen) allows fast and efficient recombination of PCR-amplified DNAs. Among TOPO vectors, a pcDNA3.1 directional cloning ...
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