[논문]Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection

Chiow, K.H.; Phoon, M.C.; Putti, T.; Tan, B.K.H.; Chow, V.T.

doi:10.1016/j.apjtm.2015.12.002

Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection 원문보기

Asian Pacific journal of tropical medicine, v.9 no.1, 2016년, pp.1 - 7

Chiow, K.H. , Phoon, M.C. , Putti, T. , Tan, B.K.H. , Chow, V.T.

Abstract ▼ AI-Helper

Objective: To evaluate the in vitro activities of the ethyl acetate (EA) fraction of Houttuynia cordata (H. cordata) Thunb. (Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). Methods: The antiviral activities of various concentrations of the EA fraction of H. cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2). Cinanserin hydrochloride was also tested against MHV. The EA fraction of H. cordata was tested for acute oral toxicity in C57BL/6 mice. Results: The EA fraction of H. cordata inhibited viral infectivity up to 6 d. Cinanserin hydrochloride was able to inhibit MHV for only 2 d. The 50% inhibitory concentrations (IC50) of the EA fraction of H. cordata added before the viral adsorption stage were 0.98 μg/mL for MHV and 7.50 μg/mL for DENV-2 with absence of cytotoxicity. The mice fed with the EA fraction up to 2000 mg/kg did not induce any signs of acute toxicity, with normal histological features of major organs. Certain flavonoids exhibited comparatively weaker antiviral activity, notably quercetin which could inhibit both MHV and DENV-2. This was followed by quercitrin which could inhibit DENV-2 but not MHV, whereas rutin did not exert any inhibitory effect on either virus. When quercetin was combined with quercitrin, enhancement of anti-DENV-2 activity and reduced cytotoxicity were observed. However, the synergistic efficacy of the flavonoid combination was still less than that of the EA fraction. Conclusions: The compounds in H. cordata contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in vivo. H. cordata has much potential for the development of antiviral agents against coronavirus and dengue infections.

주제어

참고문헌 (40)

1 Guan Y. Zheng B.J. He Y.Q. Liu X.L. Zhuang Z.X. Cheung C.L. Isolation and characterization of viruses related to the SARS coronavirus from animals in Southern China Science 302 2003 276 278 12958366

상세보기
2 Li W. Shi Z. Yu M. Ren W. Smith C. Epstein J.H. Bats are natural reservoirs of SARS-like coronaviruses Science 310 2005 676 679 16195424

상세보기
3 Lau S.K. Woo P.C. Li K.S. Huang Y. Tsoi H.W. Wong B.H. Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats Proc Natl Acad Sci U S A 102 2005 14040 14045 16169905

상세보기
4 Chow V.T. Chan Y.C. Yong R. Lee K.M. Lim L.K. Chung Y.K. Monitoring of dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing Am J Trop Med Hyg 58 1998 578 586 9598444

상세보기
5 Halstead S.B. Pathogenesis of dengue. Challenge to molecular biology Science 239 1988 476 481 3277268
6 Rico-Hesse R. Harrison L. Nisalak A. Vaughn D.W. Kalayanarooj S. Green S. Molecular evolution of dengue type 2 virus in Thailand Am J Trop Med Hyg 58 1998 96 101 9452299

상세보기
7 Vaughn D.W. Green S. Kalayanarooj S. Innis B.L. Nimmannitya S. Suntayakorn S. Dengue viremia titer, antibody response pattern, and virus serotype correlate with severity J？Infect Dis 181 2000 2 9 10608744
8 Hayashi K. Kamiya M. Hayashi T. Virucidal effects of the steam distillate from Houttuynia cordata and its components on HSV-1, influenza virus, and HIV Planta Med 61 1995 237 241 7617766

상세보기
9 Chou S.C. Su C.R. Ku Y.C. Wu T.S. The constituents and their bioactivities of Houttuynia cordata Chem Pharm Bull 57 2009 1227 1230 19881272

상세보기
10 Chen X. Wang Z. Yang Z. Wang J. Xu Y. Tan R.X. Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation Antiviral Res 92 2011 341 345 21951655
11 Kumar M. Prasad S.K. Hemalatha S. A？current update on the phytopharmacological aspects of Houttuynia cordata Thunb Pharmacogn Rev 8 2014 22 35 24600193

상세보기
12 Hung P.Y. Ho B.C. Lee S.Y. Chang S.Y. Kao C.L. Lee S.S. Houttuynia cordata targets the beginning stage of herpes simplex virus infection PLoS One 10 2015 e0115475 25643242

상세보기
13 Xie M.L. Phoon M.C. Dong S.X. Tan B.K.H. Chow V.T. Houttuynia cordata extracts and constituents inhibit the infectivity of dengue virus type 2 in？vitro Int J Integr Biol 14 2013 78 85
14 Meng J. Leung K.S. Jiang Z. Dong X. Zhao Z. Xu L.J. Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata Chem Pharm Bull 53 2005 1604 1609 16327201

상세보기
15 Havsteen B. Flavonoids, a class of natural products of high pharmacological potency Biochem Pharmacol 32 1983 1141 1148 6342623

상세보기
16 Sanchez I. Gomez-Garibay F. Taboada J. Ruiz B.H. Antiviral effect of flavonoids on the dengue virus Phytother Res 14 2000 89 92 10685103
17 Song J.M. Lee K.H. Seong B.L. Antiviral effect of catechins in green tea on influenza virus Antiviral Res 68 2005 66 74 16137775

상세보기
18 Chow V.T. Lim C.S. Phoon M.C. Tan H.C. A？seroprevalence survey of dengue virus infection in healthy Singapore university undergraduates by enzyme immunoassay and plaque reduction neutralization test Dengue Bull 29 2005 97 105
19 Lim C.S. Chua J.J. Wilkerson J. Chow V.T. Differential dengue cross-reactive and neutralizing antibody responses in BALB/c and Swiss albino mice induced by immunization with flaviviral vaccines and by infection with homotypic dengue-2 virus strains Viral Immunol 19 2006 33 41 16553548
20 Trirawatanapong T. Chandran B. Putnak R. Padmanabhan R. Mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody Gene 116 1992 139 150 1634111

상세보기
21 Wleklik M. Luczak M. Panasiak W. Kobus M. Lammer-Zarawska E. Structural basis for antiviral activity of flavonoids-naturally occurring compounds Acta Virol 32 1988 522 525 2906224

상세보기
22 Dubber M.J. Kanfer I. High-performance liquid chromatographic determination of selected flavonols in Ginkgo biloba solid oral dosage forms J？Pharm Pharm Sci 7 2004 303 309 15576009
23 Kaul T.N. Middleton E. Ogra P.L. Antiviral effect of flavonoids on human viruses J？Med Virol 15 1985 71 79 2981979

상세보기
24 Vrijsen R. Everaert L. Boeye A. Antiviral activity of flavones and potentiation by ascorbate J？Gen Virol 69 1988 1749 1751 2839607

상세보기
25 Vlietinck A.J. Vanden Berghe D.A. Haemers A. Present status and prospects of flavonoids as anti-viral agents Prog Clin Biol Res 280 1988 283 299 3051028

상세보기
26 Choi H.J. Kim J.H. Lee C.H. Ahn Y.J. Song J.H. Baek S.H. Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus Antiviral Res 81 2009 77 81 18992773

상세보기
27 Wu C.P. Calcagno A.M. Hladky S.B. Ambudkar S.V. Barrand M.A. Modulatory effects of plant phenols on human multidrug-resistance proteins 1, 4 and 5 (ABCC1, 4 and 5) FEBS J 272 2005 4725 4740 16156793

상세보기
28 Choi H.J. Song J.H. Park K.S. Kwon D.H. Inhibitory effects of quercetin 3-rhamnoside on influenza A virus replication Eur J Pharm Sci 37 2009 329 333 19491023

상세보기
29 de Sousa L.R. Wu H. Nebo L. Fernandes J.B. da Silva M.F. Kiefer W. Flavonoids as noncompetitive inhibitors of dengue virus NS2B-NS3 protease: inhibition kinetics and docking studies Bioorg Med Chem 23 2015 466 470 25564380

상세보기
30 Mucsi I. Combined antiviral effects of flavonoids and 5-ethyl-2′-deoxyuridine on the multiplication of herpesviruses Acta Virol 28 1984 395 400 6151353

상세보기
31 Lamien C.E. Meda A. Mans J. Romito M. Nacoulma O.G. Viljoen G.J. Inhibition of fowlpox virus by an aqueous acetone extract from galls of Guiera senegalensis J. F. Gmel (Combretaceae) J？Ethnopharmacol 96 2005 249 253 15588677

상세보기
32 Yang Q. Chen L. He X. Gao Z. Shen X. Bai D. Design and synthesis of cinanserin analogs as severe acute respiratory syndrome coronavirus 3CL protease inhibitors Chem Pharm Bull 56 2008 1400 1405 18827378

상세보기
33 Nguyen T.T. Woo H.J. Kang H.K. Nguyen V.D. Kim Y.M. Kim D.W. Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris Biotechnol Lett 34 2012 831 838 22350287

상세보기
34 Neuman B.W. Chamberlain P. Bowden F. Joseph J. Atlas of coronavirus replicase structure Virus Res 194 2014 49 66 24355834

상세보기
35 Tian L. Shi X. Yu L. Zhu J. Ma R. Yang X. Chemical composition and hepatoprotective effects of polyphenol-rich extract from Houttuynia cordata tea J？Agric Food Chem 60 2012 4641 4648 22515645

상세보기
36 Lau K.M. Lee K.M. Koon C.M. Cheung C.S. Lau C.P. Ho H.M. Immunomodulatory and anti-SARS activities of Houttuynia cordata J？Ethnopharmacol 118 2008 79 85 18479853

상세보기
37 Yin J. Li G. Li J. Yang Q. Ren X. In？vitro and in？vivo effects of Houttuynia cordata on infectious bronchitis virus Avian Pathol 40 2011 491 498 21848486

상세보기
38 Leardkamolkarn V. Sirigulpanit W. Phurimsak C. Kumkate S. Himakoun L. Sripanidkulchai B. The inhibitory actions of Houttuynia cordata aqueous extract on dengue virus and dengue-infected cell J？Food Biochem 36 2012 86 92
39 Sutton T.C. Subbarao K. Development of animal models against emerging coronaviruses: from SARS to MERS coronavirus Virology 479？480C 2015 247 258
40 Fu J. Dai L. Lin Z. Lu H. Houttuynia cordata Thunb: a review of phytochemistry and pharmacology and quality control Chin Med 4 2013 101 123

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (40)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트