[논문]A high-throughput mass spectrometry assay to simultaneously measure intact insulin and C-peptide

Taylor, S.W.; Clarke, N.J.; Chen, Z.; McPhaul, M.J.

doi:10.1016/j.cca.2016.01.019

A high-throughput mass spectrometry assay to simultaneously measure intact insulin and C-peptide

Clinica chimica acta, v.455, 2016년, pp.202 - 208

Taylor, S.W. , Clarke, N.J. , Chen, Z. , McPhaul, M.J.

Abstract ▼ AI-Helper

Background: Measurements of fasting levels of insulin and C-peptide are useful in documenting insulin resistance and may help predict development of diabetes mellitus. However, the specific insulin and C-peptide levels associated with specific degrees of insulin resistance have not been defined, owing to marked variability among immunoassays and lack of standardization. Herein, we describe a multiplexed liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for intact insulin and C-peptide. Methods: Insulin and C-peptide were enriched from patient sera using monoclonal antibodies immobilized on magnetic beads and processed on a robotic liquid handler. Eluted peptides were analyzed by LC-MS/MS. Bovine insulin and a stable isotopically-labeled (13C/15N) C-peptide were utilized as internal standards. Results: The assay had an analytical measurement range of 3 to 320μIU/ml (18 to 1920pmol/l) for insulin and 0.11 to 27.2ng/ml (36 to 9006pmol/l) for C-peptide. Intra- and inter-day assay variation was less than 11% for both peptides. Of the 5 insulin analogs commonly prescribed to treat diabetes, only the recombinant drug insulin lispro caused significant interference for the determination of endogenous insulin. There were no observed interferences for C-peptide. Conclusion: We developed and validated a high-throughput, quantitative, multiplexed LC-MS/MS assay for intact insulin and C-peptide.

주제어

참고문헌 (23)

Diabetes Metab. Res. Rev. Wang 26 378 2010 10.1002/dmrr.1089 Incidence rates and predictors of diabetes in those with prediabetes: the Strong Heart Study

상세보기
Endocr. Pract. Johnson 16 47 2010 10.4158/EP09031.OR Identifying prediabetes using fasting insulin levels

상세보기
Cardiovasc. Diabetol. Sung 10 108 2011 10.1186/1475-2840-10-108 Elevated fasting insulin predicts the future incidence of metabolic syndrome: a 5-year follow-up study

상세보기
Diabetes Care Dankner 32 1464 2009 10.2337/dc09-0153 Basal-state hyperinsulinemia in healthy normoglycemic adults is predictive of type 2 diabetes over a 24-year follow-up: a preliminary report

상세보기
Diabetes Metab. Res. Rev. Dankner 28 618 2012 10.1002/dmrr.2322 Basal state hyperinsulinemia in healthy normoglycemic adults heralds dysglycemia after more than two decades of follow up

상세보기
Clin. Chem. Manley 53 922 2007 10.1373/clinchem.2006.077784 Comparison of 11 human insulin assays: implications for clinical investigation and research

상세보기
Clin. Chem. Marcovina 53 711 2007 10.1373/clinchem.2006.082214 Standardization of insulin immunoassays: report of the American Diabetes Association workgroup

상세보기
BMJ Stratton 321 405 2000 10.1136/bmj.321.7258.405 Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35): prospective observational study

상세보기
Clin. Chem. Chen 59 1349 2013 10.1373/clinchem.2012.199794 Quantitative insulin analysis using liquid chromatography-tandem mass spectrometry in a high-throughput clinical laboratory

상세보기
Anal. Chem. Chambers 86 694 2014 10.1021/ac403055d Multidimensional LC-MS/MS enables simultaneous quantification of intact human insulin and five recombinant analogs in human plasma

상세보기
Proteomics Peterman 14 1445 2014 10.1002/pmic.201300300 An automated, high-throughput method for targeted quantification of intact insulin and its therapeutic analogs in human serum or plasma coupling mass spectrometric immunoassay with high resolution and accurate mass detection (MSIA-HR/AM)

상세보기
Drug Test Anal. Thomas 6 1125 2014 10.1002/dta.1710 Determination of human insulin and its analogues in human blood using liquid chromatography coupled to ion mobility mass spectrometry (LC-IM-MS)

상세보기
Clin. Chem. Cabaleiro 52 1193 2006 10.1373/clinchem.2005.062505 Feasibility of standardization of serum C-peptide immunoassays with isotope-dilution liquid chromatography-tandem mass spectrometry

상세보기
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Kinumi 953-954 138 2014 10.1016/j.jchromb.2014.02.019 Quantification of serum C-peptide by isotope-dilution liquid chromatography-tandem mass spectrometry: enhanced detection using chemical modification and immunoaffinity purification

상세보기
Clin. Diagn. Lab. Immunol. Castro 7 197 2000 10.1128/CDLI.7.2.197-199.2000 Lipid removal from human serum samples

상세보기
Clin. Chem. Lab. Med. Heurtault 52 355 2014 10.1515/cclm-2013-0427 Extensive study of human insulin immunoassays: promises and pitfalls for insulin analogue detection and quantification

상세보기
J. Clin. Endocrinol. Metab. Cryer 94 709 2009 10.1210/jc.2008-1410 Evaluation and management of adult hypoglycemic disorders: an Endocrine Society Clinical Practice Guideline

상세보기
Bioanalysis Chambers 5 65 2013 10.4155/bio.12.290 Development of a fast method for direct analysis of intact synthetic insulins in human plasma: the large peptide challenge

상세보기
Clin. Chem. Lab. Med. Haeckel 42 1069 2004 10.1515/CCLM.2004.215 Reference interval of serum insulin concentrations

상세보기
Clin. Chem. Lab. Med. Schultess 47 834 2009 10.1515/CCLM.2009.185 Diagnostic performance of the ARCHITECT C-peptide immunoassay

상세보기
Diabet. Med. Jones 30 803 2013 10.1111/dme.12159 The clinical utility of C-peptide measurement in the care of patients with diabetes

상세보기
National Institute for Biological Standards and Control (NIBSC). C-peptide of human insulin, International Reference Reagent NIBSC code: 84/510. Instructions for use. Version 5.0. http://www.nibsc.org/documents/ifu/84-510.pdf. Updated December 2013. Accessed December 2015.
Medecine Nucleaire Van Uytfanghe 34 566 2010 10.1016/j.mednuc.2010.07.005 Standardization of insulin and C-peptide - a status report

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

A high-throughput mass spectrometry assay to simultaneously measure intact insulin and C-peptide

Abstract ▼ AI-Helper

주제어

참고문헌 (23)

이 논문을 인용한 문헌

관련 콘텐츠

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트