[논문]LC-MS/MS method for quantitation of seven biomarkers in human plasma for the assessment of insulin resistance and impaired glucose tolerance

Zhang, Q.; Ford, L.A.; Goodman, K.D.; Freed, T.A.; Hauser, D.M.; Conner, J.K.; Vroom, K.E.T.; Toal, D.R.

doi:10.1016/j.jchromb.2016.10.025

LC-MS/MS method for quantitation of seven biomarkers in human plasma for the assessment of insulin resistance and impaired glucose tolerance

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, v.1038, 2016년, pp.101 - 108

Zhang, Q. , Ford, L.A. , Goodman, K.D. , Freed, T.A. , Hauser, D.M. , Conner, J.K. , Vroom, K.E.T. , Toal, D.R.

Abstract ▼ AI-Helper

Early detection of insulin resistance (IR) and/or impaired glucose tolerance (IGT) is crucial for delaying and preventing the progression toward type 2 diabetes. We recently developed and validated a straightforward metabolite-based test for the assessment of IR and IGT in a single LC-MS/MS method. Plasma samples were diluted with isotopically-labeled internal standards and extracted by simple protein precipitation. The extracts were analyzed by LC-MS/MS for the quantitation of 2-hydroxybutyric acid (0.500-40.0μg/mL), 3-hydroxybutyric acid (1.00-80.0μg/mL), 4-methyl-2-oxopentanoic acid (0.500-20.0μg/mL), 1-linoleoyl-2-hydroxy-sn-glycero-3-phosphocholine (2.50-100μg/mL), oleic acid (10.0-400μg/mL), pantothenic acid (0.0100-0.800μg/mL), and serine (2.50-100μg/mL). Liquid chromatography was carried out on a reversed phase column with a run time of 3.1min and the mass spectrometer operated in negative MRM mode. Method validation was performed on three identical LC-MS/MS systems with five runs each. Sufficient linearity (R2>0.99) was observed for all the analytes over the ranges. The imprecision (CVs) was found to be less than 5.5% for intra-run and less than 5.8% for inter-run for the seven analytes. The analytical recovery was determined to be between 96.3 and 103% for the seven analytes. This fast and robust method has subsequently been used for patient sample analysis for the assessment of IR and IGT.

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LC-MS/MS method for quantitation of seven biomarkers in human plasma for the assessment of insulin resistance and impaired glucose tolerance

Abstract ▼ AI-Helper

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참고문헌 (21)

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