Abstract Rice husk as an abundant biomass was used in this study, and it contained 30.1% glucan and 13.5% xylan, 22.4% lignin. The pretreated rice husk with glycerol carbonate and acidified aqueous glycerol (10% water) at 90°C and 130°C for 60min had the maximum yield of glucan digestibility which was 78.2% and 69.7% respectively, using cellulase for 72h. The simultaneous saccharification and fermentation was conducted anaerobically at 37°C with Saccharomyces cerevisiae, 5% w/v glucan and 10FPU/g glucan of cellulase. 11.58 and 8.84g/L was the highest ethanol concentration after 3days of incubation form pretreated rice husk with glycerol carbonate and acidified aqueous glycerol respectively. Highlights Glycerol carbonate solvent as a novel pretreatment was performed on rice husk. A small portion of acids as a catalyst mixed with the pretreatment solvents. AGC treatment improved glucan digestibility and lignin removal more than AAG treatment. Ethanol production of 11.58 and 8.84g/L obtained from SSF under anaerobic condition.
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