[논문]The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing

Yeo, Min-Kyung; Jung, Min-Kyu; Lee, Su-Yel; Lee, Yong-Moon; Hur, Gang Min; Kim, Jin-Man

doi:10.1136/jclinpath-2016-204025

The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing

Journal of clinical pathology, v.70 no.3, 2017년, pp.260 - 265

Yeo, Min-Kyung (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea) , Jung, Min-Kyu (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea) , Lee, Su-Yel (Biobank of Chungnam National University Hospital, , Daejeon, Korea) , Lee, Yong-Moon (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea) , Hur, Gang Min (Department of Pharmacology, Chungnam National University School of Medicine, , Daejeon, Korea) , Kim, Jin-Man (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea)

Abstract ▼ AI-Helper

Introduction The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%-87% of PTC by various molecular methods. Aim(s) A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. Methods 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. Results The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. Conclusions This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations.

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참고문헌 (23)

Oncogene Dhillon 26 3279 2007 10.1038/sj.onc.1210421 MAP kinase signalling pathways in cancer

상세보기
Ann Transl Med Karachaliou 3 265 2015 Melanoma: oncogenic drivers and the immune system
Proc Natl Acad Sci USA Franco 108 1615 2011 10.1073/pnas.1015557108 Thyrotrophin receptor signaling dependence of Braf-induced thyroid tumor initiation in mice

상세보기
Endocr Relat Cancer Xing 12 245 2005 10.1677/erc.1.0978 BRAF mutation in thyroid cancer

상세보기
Int J Cancer Gandolfi 137 1001 2015 10.1002/ijc.28976 Time to re-consider the meaning of BRAF V600E mutation in papillary thyroid carcinoma

상세보기
Ann Surg Oncol Jeong 20 759 2013 10.1245/s10434-012-2494-0 BRAF (V600E) mutation analysis in papillary thyroid carcinomas by peptide nucleic acid clamp real-time PCR

상세보기
Yonsei Med J Kim 45 818 2004 10.3349/ymj.2004.45.5.818 Mutations of the BRAF gene in papillary thyroid carcinoma in a Korean population

상세보기
Thyroid Jung 22 791 2012 10.1089/thy.2011.0123 Mutational patterns and novel mutations of the BRAF gene in a large cohort of Korean patients with papillary thyroid carcinoma

상세보기
JAMA Otolaryngol Head Neck Surg Davies 140 317 2014 10.1001/jamaoto.2014.1 Current thyroid cancer trends in the United States

상세보기
Ann Surg Oncol Toniato 15 1518 2008 10.1245/s10434-008-9859-4 Papillary thyroid carcinoma: factors influencing recurrence and survival

상세보기
Am J Med Mazzaferri 97 418 1994 10.1016/0002-9343(94)90321-2 Long-term impact of initial surgical and medical therapy on papillary and follicular thyroid cancer

상세보기
Eur J Clin Invest Wang 46 146 2016 10.1111/eci.12577 Clinical impact of BRAF mutation on the diagnosis and prognosis of papillary thyroid carcinoma: a systematic review and meta-analysis

상세보기
J Korean Med Sci Cho 29 1054 2014 10.3346/jkms.2014.29.8.1054 Clinicopathological features of rare BRAF mutations in Korean thyroid cancer patients

상세보기
Oncogene Fukushima 22 6455 2003 10.1038/sj.onc.1206739 BRAF mutations in papillary carcinomas of the thyroid

상세보기
Korean J Pathol Jeong 46 61 2012 10.4132/KoreanJPathol.2012.46.1.61 Detection of BRAF(V600E) mutations in papillary thyroid carcinomas by peptide nucleic acid clamp real-time PCR: a comparison with direct sequencing

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Am J Clin Pathol Kang 139 759 2013 10.1309/AJCPN3ULH6YWBHPH Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clamping and real-time PCR

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Am J Clin Pathol Lee 133 802 2010 10.1309/AJCPO3F2ENKMDTUS Detection of BRAF mutations in thyroid nodules by allele-specific PCR using a dual priming oligonucleotide system

상세보기
Health Technol Assess Snowsill 18 1 2014 10.3310/hta18580 A systematic review and economic evaluation of diagnostic strategies for Lynch syndrome

상세보기
Oncotarget Janku 6 26886 2015 10.18632/oncotarget.4723 BRAF mutation testing with a rapid, fully integrated molecular diagnostics system

상세보기
Exp Mol Pathol Melchior 99 485 2015 10.1016/j.yexmp.2015.09.004 Multi-center evaluation of the novel fully-automated PCR-based Idylla BRAF Mutation Test on formalin-fixed paraffin-embedded tissue of malignant melanoma

상세보기
Onco Targets Ther Spagnolo 8 157 2015 10.2147/OTT.S39096 BRAF-mutant melanoma: treatment approaches, resistance mechanisms, and diagnostic strategies

상세보기
Thyroid Falchook 25 71 2015 10.1089/thy.2014.0123 BRAF inhibitor dabrafenib in patients with metastatic BRAF-mutant thyroid cancer

상세보기
J Gastrointest Oncol Corcoran 6 650 2015 New therapeutic strategies for BRAF mutant colorectal cancers

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The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing

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The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing

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