Abstract Comprehensive utilization of medicinal plant resources is of great significance for sustainable development of traditional Chinese medicines. In the present study, the α-glucosidase inhibitory activities of the rhizome and fibrous root of Anemarrhena Asphodeloides Bunge, were compare...
Abstract Comprehensive utilization of medicinal plant resources is of great significance for sustainable development of traditional Chinese medicines. In the present study, the α-glucosidase inhibitory activities of the rhizome and fibrous root of Anemarrhena Asphodeloides Bunge, were compared detailedly, and a high performance liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-QQQ/MS) method was developed for simultaneous quantification of seven bioactive constituents including neomangiferin, mangiferin, isomangiferin, timosaponin BII, timosaponin B, timosaponin AIII, and timosaponin N in 40 batches of samples. The results demonstrated that fibrous root extracts had more potent α-glucosidase inhibitory activity than rhizome extracts. Mangiferin and isomangiferin were abundant in fibrous root, while the analyzed saponins were rich in rhizome. Based on the chemometrics methods including principal component analysis (PCA), orthogonal partial least square discriminant analysis (OPLS-DA), and partial least square (PLS), mangiferin and isomangiferin might be mainly responsible for α-glucosidase inhibitory activity of the genus. These findings indicate that the established HPLC-QQQ/MS method was proven to be useful and efficient for quality control of Anemarrhena materials, and fibrous root had the potential to be utilized as anti-diabetic medicinal resource. Highlights The α-glucosidase inhibitory effect of AR and AFR were compared. LC–MS/MS method was developed for determination of 7 bioactive constituents. Chemometrics methods were employed to deal with the quantitative date. AFR had the potential to be used as anti-diabetic medicinal resource. Graphical abstract [DISPLAY OMISSION]
Abstract Comprehensive utilization of medicinal plant resources is of great significance for sustainable development of traditional Chinese medicines. In the present study, the α-glucosidase inhibitory activities of the rhizome and fibrous root of Anemarrhena Asphodeloides Bunge, were compared detailedly, and a high performance liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-QQQ/MS) method was developed for simultaneous quantification of seven bioactive constituents including neomangiferin, mangiferin, isomangiferin, timosaponin BII, timosaponin B, timosaponin AIII, and timosaponin N in 40 batches of samples. The results demonstrated that fibrous root extracts had more potent α-glucosidase inhibitory activity than rhizome extracts. Mangiferin and isomangiferin were abundant in fibrous root, while the analyzed saponins were rich in rhizome. Based on the chemometrics methods including principal component analysis (PCA), orthogonal partial least square discriminant analysis (OPLS-DA), and partial least square (PLS), mangiferin and isomangiferin might be mainly responsible for α-glucosidase inhibitory activity of the genus. These findings indicate that the established HPLC-QQQ/MS method was proven to be useful and efficient for quality control of Anemarrhena materials, and fibrous root had the potential to be utilized as anti-diabetic medicinal resource. Highlights The α-glucosidase inhibitory effect of AR and AFR were compared. LC–MS/MS method was developed for determination of 7 bioactive constituents. Chemometrics methods were employed to deal with the quantitative date. AFR had the potential to be used as anti-diabetic medicinal resource. Graphical abstract [DISPLAY OMISSION]
J. Ethnopharmacol. Kang 131 485 2010 10.1016/j.jep.2010.07.025 The analgesic and anti-inflammatory effect of WIN-34B, a new herbal formula for osteoarthritis composed of Lonicera japonica Thunb and Anemarrhena asphodeloides Bunge. in vivo
Food Chem. Xiong 138 327 2013 10.1016/j.foodchem.2012.10.127 Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves
Steroids Zhang 89 1 2014 10.1016/j.steroids.2014.07.002 Steroidal sapogenins and glycosides from the fibrous roots of Polygonatum Odoratum with inhibitory effect on tissue factor (TF) procoagulant activity
Steroids Sun 92 90 2014 10.1016/j.steroids.2014.09.008 Steroidal saponins with induced platelet aggregation activity from the aerial parts of Paris verticillata
2015 The State Commission of Chinese Pharmacopoeia, Pharmacopoeia of Peoples Republic of China
J. Ethnopharmacol. Wang 153 42 2014 10.1016/j.jep.2014.02.013 The genus Anemarrhena Bunge: a review on ethnopharmacology, phytochemistry and pharmacology
Chin. Tradit. Herbal Drugs Ji 41 appendix11 2010 Advances in studies on saponins in Anemarrhena asphodeloides
J. Diabetes Zhao 4 304 2014 10.1111/1753-0407.12111 Total polyphenol of Anemarrhena asphodeloides ameliorates advanced glycation end products-induced endothelial dysfunction by regulation of AMP-Kinase
J. Ethnopharmacol. Han 172 368 2015 10.1016/j.jep.2015.05.016 Rhizoma Anemarrhenae extract ameliorates hyperglycemia and insulin resistance via activation of AMP-activated protein kinase in diabetic rodent
Int. Immunopharmacol. Lim 34 220 2016 10.1016/j.intimp.2016.03.004 Mangiferin corrects the imbalance of Th17/Treg cells in mice with TNBS-induced colitis
Cancer Res. Sy 68 10229 2008 10.1158/0008-5472.CAN-08-1983 Timosaponin AIII induces autophagy preceding mitochondria-mediated apoptosis in HeLa cancer cells
Phytochem. Lett. Sun 7 207 2014 10.1016/j.phytol.2013.12.005 Two new components from the rhizome of Anemarrhena asphodeloides Bge. and their antiplatelet aggregative activity
Clin. Exp. Pharmacol. Physiol. Lu 38 430 2011 10.1111/j.1440-1681.2011.05530.x Antiplatelet and antithrombotic activities of timosaponin BII, an extract of Anemarrhena asphodeloides
Chem. Biol. Interact. Agustini 240 22 2015 10.1016/j.cbi.2015.08.001 Cardioprotective effects of timosaponin B II from Anemarrhenae asphodeloides Bge on isoproterenol-induced myocardial infarction in rats
Lat. Am. J. Pharm. Zhou 4 1129 2015 Mangiferin improves memory deficits followed cerebral ischemia reperfusion in mice through attenuation of oxidative stress and AChE activity
Zhongguo Zhong Yao Za Zhi Liu 24 554 1999 Determination of sarsasapogenin in Anemarrhena asphodeloides Bunge by GC
J. Med. Plants Res Chen 6 1585 2012 Determination of two major xanthone glycosides in rhizome of Anemarrhena asphodeloides using high performance capillary electrophoresis
Pharm. J. Chin. PLA. Zhai 23 472 2007 Determination of mangiferin and neomangiferin in Rhizoma Anemarrhenae and its preparation by HPLC
Chin. Tradit. Herbal Drugs Zhong 47 2747 2016 Research on HPLC-ELSD fingerprint of anemarrhenae rhizoma
Anal. Lett. Zhang 43 2210 2010 10.1080/00032711003717307 Identification and simultaneous determination of Mangiferin, Neomangiferin Timosaponin A-III, and Timosaponin C in Rhizoma anemarrhenae by rapid resolution liquid chromatography coupled with triple quadrupole mass spectrometry
J. Sep. Sci. Sun 35 1796 2012 10.1002/jssc.201200026 Simultaneous determination of nine components in Anemarrhena asphodeloides by liquid chromatography-tandem mass spectrometry combined with chemometric techniques
J. Pharm. Biomed. Anal. Kim 92 47 2014 10.1016/j.jpba.2013.12.040 A metabolomic approach to determine the geographical origins of Anemarrhena asphodeloides by using UPLC-QTOF MS
Steroids Liu 96 21 2015 10.1016/j.steroids.2015.01.006 Development and validation of liquid chromatography?tandem mass spectrometry method for simultaneous determination of six steroidal saponins in rat plasma and its application to a pharmacokinetics study
J. Pharm. Biomed. Anal. Guo 117 91 2016 10.1016/j.jpba.2015.08.038 Comparative analysis of steroidal saponins in four Dioscoreae herbs by high performance liquid chromatography coupled with mass spectrometry
J. Pharm. Biomed. Anal. Bhatt 132 46 2017 10.1016/j.jpba.2016.09.035 Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS
J. Pharm. Biomed. Anal. Hsu 132 109 2017 10.1016/j.jpba.2016.09.027 Simultaneous determination of phenolic acids and flavonoids in Chenopodium formosanum Koidz. (djulis) by HPLC-DAD-ESI-MS/MS
BioMed. Res. Int. Brindis 591313 2013 Aqueous extract of Annona macroprophyllata: a potential alpha-glucosidase inhibitor
Molecules Yan 21 2016 10.3390/molecules21091148 Purification of flavonoids from chinese bayberry (Morella rubra sieb. et zucc.) fruit extracts and alpha-Glucosidase inhibitory activities of different fractionations
Biomed. Chromatogr.: BMC Zhao 27 1621 2013 10.1002/bmc.2970 Screening and determination for potential alpha-glucosidase inhibitory constituents from Dalbergia odorifera T. Chen using ultrafiltration-LC/ESI-MS(n)
Molecules Sheng 19 10563 2014 10.3390/molecules190710563 Isolation and characterization of an alpha-glucosidase inhibitor from Musa spp. (Baxijiao) flowers
J. Chromatogr. B Xie 1026 134 2016 10.1016/j.jchromb.2015.12.003 Metabolism and pharmacokinetics of major polyphenol components in rat plasma after oral administration of total flavonoid tablet from Anemarrhenae Rhizoma
Braz. J. Biol. Habermann 76 898 2016 10.1590/1519-6984.03815 Antioxidant activity and phenol content of extracts of bark stems, and young and mature leaves from Blepharocalyx salicifolius (Kunth) O. Berg
Am. J. Chin. Med. Yuan 44 755 2016 10.1142/S0192415X16500415 Timosaponin BII ameliorates palmitate-induced insulin resistance and inflammation via IRS-1/PI3K/Akt and IKK/NF-κ B pathways
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