[논문]Fecal bacteria from treatment-naive Crohn's disease patients can skew helper T cell responses

Ma, Fei; Zhang, Yi; Xing, Junjie; Song, Xiaoling; Huang, Ling; Weng, Hao; Wu, Xiangsong; Walker, Emma; Wang, Zhongchuan

doi:10.1016/j.yexcr.2017.10.011

Fecal bacteria from treatment-naive Crohn's disease patients can skew helper T cell responses

Experimental cell research, v.361 no.1, 2017년, pp.135 - 140

Ma, Fei (Department of Oncology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine) , Zhang, Yi (Department of Digestive Endoscopic Diagnosis and Treatment, Xinhua Hospital, Shanghai Jiaotong University School of Medicine) , Xing, Junjie (Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University) , Song, Xiaoling (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine) , Huang, Ling (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine) , Weng, Hao (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine) , Wu, Xiangsong (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine) , Walker, Emma (DICAT Biomedical Computation Centre) , Wang, Zhongchuan (Department of Colorectal Surgery, Xinhua Hospital)

Abstract ▼ AI-Helper

Abstract Many studies have demonstrated that the inflamed mucosa of Crohn's disease (CD) patients presented a disturbed gut commensal community, and the shift in microbial composition and species variety is associated with disease severity. To establish a link between changes in the intestinal bacterial composition and the alteration of inflammation, we obtained fecal bacteria from CD patients and non-CD controls. The bacteria were then used to stimulate the peripheral blood mononuclear cells (PBMCs) from one non-CD individual. We found that the frequency of IFN-γ- and IL-17-expressing CD4 T cells was significantly higher after stimulation with CD bacteria than with non-CD bacteria, while the frequency of IL-4- and IL-10-expressing CD4 T cells was significantly decreased after stimulation with CD bacteria. A similar trend was observed in the level of cytokine expression and transcription expression. However, this difference was not clear-cut, as overlapping regions were observed between the two groups. With longer stimulation using CD bacteria, the skewing toward Th1/Th17 responses were further increased. This increase depended on the presence of monocytes/macrophages. Interestingly, we also found that B cells presented an inhibitory effect in CD bacteria-mediated skewing toward Th1/Th17 cells and promoted IL-10 secretion in CD bacteria-stimulated PBMCs. Together, our results demonstrated that CD bacteria could promote Th1/Th17 inflammation in a host factor-independent fashion.

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Fecal bacteria from treatment-naive Crohn's disease patients can skew helper T cell responses

Abstract ▼ AI-Helper

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참고문헌 (19)

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