[논문]Molecular characterization of Cryptococcus neoformans and Cryptococcus gattii from environmental sources and genetic comparison with clinical isolates in Apulia, Italy

Montagna, Maria Teresa; De Donno, Antonella; Caggiano, Giuseppina; Serio, Francesca; De Giglio, Osvalda; Bagordo, Francesco; D’Amicis, R.; Lockhart, Shawn R.; Cogliati, Massimo

doi:10.1016/j.envres.2017.09.032

Molecular characterization of Cryptococcus neoformans and Cryptococcus gattii from environmental sources and genetic comparison with clinical isolates in Apulia, Italy 원문보기

Environmental research, v.160, 2018년, pp.347 - 352

Montagna, Maria Teresa (Department of Biomedical Science and Human Oncology, University of Bari 'Aldo Moro', Bari, Italy) , De Donno, Antonella (Department of Biological and Environmental Science and Technology, University of Salento, Lecce, Italy) , Caggiano, Giuseppina (Department of Biomedical Science and Human Oncology, University of Bari 'Aldo Moro', Bari, Italy) , Serio, Francesca (Department of Biological and Environmental Science and Technology, University of Salento, Lecce, Italy) , De Giglio, Osvalda (Department of Biomedical Science and Human Oncology, University of Bari 'Aldo Moro', Bari, Italy) , Bagordo, Francesco (Department of Biological and Environmental Science and Technology, University of Salento, Lecce, Italy) , D’Amicis, R. (Department of Biomedical Sciences for Health, Università) , Lockhart, Shawn R. (degli Studi di Milano, Milano, Italy) , Cogliati, Massimo (Fungal Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, GA, USA)

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Abstract The present study investigated the environmental distribution of Cryptococcus neoformans and C. gattii species complex molecular types, mating types and sequence types in Apulia, a region of Southern Italy. A total of 2078 specimens from arboreal and animal sources were analyzed. The percentage of positive samples was similar among both arboreal and animal specimens: 4.2% vs. 5.1% for C. neoformans species complex and 0.6% vs. 1.4% for C. gattii species complex. Molecular typing identified 78 isolates as VNI (76 αA and two aA), one as AD-hybrid αADa, and 16 as VGI aB. VNI isolates presented 10 different sequence types (STs) and VGI isolates two. The most frequent STs among C. neoformans and C. gattii species complex isolates were ST23 (51%) and ST156 (90%), respectively. Comparison with molecular types and STs results obtained from 21 clinical isolates collected in Apulia showed that one C. neoformans VNI clinical isolate shared an identical sequence type of one arboreal isolate (ST61) and that one C. gattii VGI clinical isolate matched with the main ST (ST156) present in the environment. In addition, molecular type VNIV was found only among clinical isolates and was absent in the investigated environmental area. In conclusion, the present study identified which C. neoformans and C. gattii species complex genotypes are circulating in Apulia, defined their ecological niches and revealed the relationship with clinical cases. It represents a basal study for addressing future investigations and public health interventions in the region. Highlights Meta-analysis of data recorded during environmental surveys of Cryptococcus spp in Apulia. Olive trees can be an alternative ecological niche for C. neoformans and C. gattii. Correlation clinical cases with possible new environmental sources of infection.

참고문헌 (31)

Mycopathologia Bartlett 173 5-6 311 2012 10.1007/s11046-011-9475-x A decade of experience: cryptococcus gattii in British Columbia

상세보기
Med. Mycol. Cafarchia 44 6 485 2006 10.1080/13693780600735452 Role of birds of prey as carriers and spreaders of Cryptococcus neoformans and other zoonotic yeasts

상세보기
Clin. Microbiol. Rev. Chen 27 980 2014 10.1128/CMR.00126-13 Cryptococcus gattii infections

상세보기
Scientifica Cogliati 2013 675213 2013 10.1155/2013/675213 Global molecular epidemiology of Cryptococcus neoformans and Cryptococcus gattii: an atlas of the molecular types

상세보기
Med. Mycol. Cogliati 38 2 97 2000 10.1080/mmy.38.2.97.103 Genotyping Cryptococcus neoformans var. neoformans with specific primers designed from PCR-fingerprinting bands sequenced using a modified PCR-based strategy

상세보기
Med. Mycol. Cogliati 51 5 499 2013 10.3109/13693786.2012.751642 Molecular epidemiology of Italian clinical Cryptococcus neoformans var. grubii isolates

상세보기
Clin. Microbiol. Infect. Cogliati 21 190 e1 2015 Cryptococcus gattii sero-mating type allelic pattern determined by multiplex PCR
Fungal Genet. Biol. Cogliati 87 22 2016 10.1016/j.fgb.2016.01.003 Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population

상세보기
FEMS Yeast Res. Cogliati 16 7 fow086 2016 10.1093/femsyr/fow086 Environmental distribution of Cryptococcus neoformans and C. gattii around the Mediterranean basin

상세보기
J. Clin. Microbiol. Ellis 28 1642 1990 10.1128/JCM.28.7.1642-1644.1990 Natural habitat of Cryptococcus neoformans var. gattii

상세보기
Clin. Microbiol. Infect. Esposto 10 12 1092 2004 10.1111/j.1469-0691.2004.00972.x Determination of Cryptococcus neoformans var. neoformans mating type by multiplex PCR

상세보기
J. Clin. Microbiol. Feng 51 3110 2013 10.1128/JCM.01455-13 Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay

상세보기
Med. Mycol. FIMUA Cryptococcosis Network 40 5 507 2002 10.1080/mmy.40.5.507.517 European Confederation of Medical Mycology (ECMM) prospective survey of cryptococcosis: report from Italy

상세보기
Emerg. Infect. Dis. Hagen 18 1618 2012 10.3201/eid1810.120068 Autochthonous and dormant Cryptococcus gattii infections in Europe

상세보기
Fungal Genet. Biol. Hagen 78 16 2015 10.1016/j.fgb.2015.02.009 Recognition of seven species in the Cryptococcus neoformans/C. gattii species complex

상세보기
Heitman 2011 Cryptococcus: From Human Pathogen to Model Yeast
Mycopathologia Iatta 174 1 87 2012 10.1007/s11046-011-9493-8 Cryptococcus gattii infection in an immunocompetent patient from Southern Italy

상세보기
Med. Mycol. Iatta 53 666 2015 10.1093/mmy/myv045 Cryptococcus neoformans in the respiratory tract of squirrels, Callosciurus finlaysonii (Rodentia, Sciuridae)

상세보기
Proc. Natl. Acad. Sci. USA Kidd 101 17258 2004 10.1073/pnas.0402981101 A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada)

상세보기
mSphere Kwon-Chung 2 1 2017 10.1128/mSphere.00357-16 The case for adopting the “species complex” nomenclature for the etiologic agents of cryptococcosis

상세보기
Vet. Microbiol Maestrale 177 409 2015 10.1016/j.vetmic.2015.03.008 Genetic and pathological characteristics of Cryptococcus gattii and Cryptococcus neoformans var. neoformans from meningoencephalitis in autochthonous goats and mouflons, Sardinia, Italy

상세보기
Emerg. Infect. Dis. Meyer 9 2 189 2003 10.3201/eid0902.020246 Molecular typing of IberoAmerican Cryptococcus neoformans isolates

상세보기
Med. Mycol. Meyer 47 561 2009 10.1080/13693780902953886 Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

상세보기
Riv. Ital. Ig. Montagna 56 69 1996 Criptococcosi e AIDS. Nota I: Indagini sulla diffusione di Cryptococcus neoformans nelle citta di Bari e Lecce
J. Mycol. Med. Montagna 7 90 1997 Cryptococcus neoformans var. gattii en Italie. Note I. Premier cas autochtone de me´ningite de se´rotype B chez un sujet VIH positif
J. Mycol. Med Montagna 7 93 1997 Cryptococcus neoformans var. gattii in Italy. Note II. environmental investigation related to an autochtonous clinical case in Apulia
Med. Mycol. Montagna 41 5 451 2003 10.1080/13693780310001602785 Cavernicolous habitats harbouring Cryptococcus neoformans: results of a speleological survey in Apulia, Italy, 1999-2000

상세보기
Lancet Infect. Dis. Rajasingham 17 8 873 2017 10.1016/S1473-3099(17)30243-8 Global burden of disease of HIV-associated cryptococcal meningitis: an updated analysis

상세보기
Mycopathologia Romeo 171 6 423 2011 10.1007/s11046-010-9389-z Environmental isolation of Cryptococcus gattii serotype B, VGI/MATα strains in southern Italy

상세보기
Mycopathologia Romeo 174 4 283 2012 10.1007/s11046-012-9547-6 Cryptococcus neoformans/Cryptococcus gattii species complex in southern Italy: an overview on the environmental diffusion of serotypes, genotypes and mating-types

상세보기
Emerg. Infect. Dis. Springer 16 14 2010 10.3201/eid1601.090369 Projecting global occurrence of Cryptococcus gattii

상세보기

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Molecular characterization of Cryptococcus neoformans and Cryptococcus gattii from environmental sources and genetic comparison with clinical isolates in Apulia, Italy 원문보기

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