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논문 상세정보

Molecular cloning and characterization under different stress conditions of insulin-like peptide 2 gene (AccILP-2) from Apis cerana cerana

Journal of asia-pacific entomology v.21 no.2 , 2018년, pp.474 - 481  
Abstract

Abstract Insulin-like peptide 2 is an important gene in the insulin/insulin-like signaling pathway. The insulin-like peptide 2 gene (ILP-2) appears to play a key role in metabolism, growth, reproduction, stress resistance and aging. In this study, we isolated and characterized an ILP-2 gene from Apis cerana cerana known as AccILP-2. The full-length AccILP-2 cDNA is 494 bp and contains a 222-bp open reading frame (ORF) encoding a protein of 73 amino acids with a calculated molecular weight of 8.706 kDa and an isoelectric point of 9.02. Multiple sequence alignment revealed that AccILP-2 shares high identity with ILP genes from Apis mellifera, Apis florea, Bombus terrestris, Eufriesea mexicana and Dufourea novaeangliae and contains a typical IlGF-insulin-bombyxin-like domain. Quantitative PCR analysis indicated that the greatest expression of AccILP-2 was observed in the muscles of adult workers. Expression analysis results also indicated that AccILP-2 expression was greatest during the dark-eye developmental period. Furthermore, expression of the AccILP-2 transcript was induced by environmental stressors, including exposure to 4 °C, ultraviolet light, H2O2, heavy metals (HgCl2 and CdCl2) and pesticides (dichlorvos, paraquat and cyhalothrin). However, AccILP-2 expression was downregulated at 44 °C. In conclusion, these results suggested that AccILP-2 might play an important role in the response to abiotic stress. We expect that our study will promote future research on the function of the insulin/insulin-like signaling pathway. Highlights The full-length AccILP-2 cDNA is 494 bp and contains a 222-bp open reading frame (ORF). The greatest expression of AccILP-2 was observed in the muscles of adult workers. AccILP-2 expression was greatest during the dark-eye developmental period. AccILP-2 might play an important role in the response to abiotic stress. Graphical abstract [DISPLAY OMISSION]

  

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