[논문]Antioxidant Effect of Barley Sprout Extract via Enhancement of Nuclear Factor-Erythroid 2 Related Factor 2 Activity and Glutathione Synthesis

Lee, Yun-Hee; Kim, Sou Hyun; Lee, Seunghyun; Kim, Kyung-Mi; Jung, Jae-Chul; Son, Tae Gen; Ki, Sung Hwan; Seo, Woo-Duck; Kwak, Jae-Hwan; Hong, Jin Tae; Jung, Young-Suk

doi:10.3390/nu9111252

Antioxidant Effect of Barley Sprout Extract via Enhancement of Nuclear Factor-Erythroid 2 Related Factor 2 Activity and Glutathione Synthesis 원문보기

Nutrients, v.9 no.11, 2017년, pp.1252 -

Lee, Yun-Hee (College of Pharmacy, Yonsei University, Incheon 21983, Korea) , Kim, Sou Hyun (yunhee.lee@yonsei.ac.kr) , Lee, Seunghyun (College of Pharmacy, Pusan National University, Busan 46241, Korea) , Kim, Kyung-Mi (hyunie9808@naver.com (S.H.K.)) , Jung, Jae-Chul (tmdgus4374@naver.com (S.L.)) , Son, Tae Gen (College of Pharmacy, Pusan National University, Busan 46241, Korea) , Ki, Sung Hwan (hyunie9808@naver.com (S.H.K.)) , Seo, Woo-Duck (tmdgus4374@naver.com (S.L.)) , Kwak, Jae-Hwan (Life Science Research Institute, Novarex Co., Ltd., Ochang, Cheongju 28126, Korea) , Hong, Jin Tae (kkm3507@novarex.co.kr (K.-M.K.)) , Jung, Young-Suk (jcjung@novarex.co.kr (J.-C.J.))

Abstract ▼ AI-Helper

We previously showed that barley sprout extract (BSE) prevents chronic alcohol intake-induced liver injury in mice. BSE notably inhibited glutathione (GSH) depletion and increased inflammatory responses, revealing its mechanism of preventing alcohol-induced liver injury. In the present study we investigated whether the antioxidant effect of BSE involves enhancing nuclear factor-erythroid 2 related factor 2 (Nrf2) activity and GSH synthesis to inhibit alcohol-induced oxidative liver injury. Mice fed alcohol for four weeks exhibited significantly increased oxidative stress, evidenced by increased malondialdehyde (MDA) level and 4-hydroxynonenal (4-HNE) immunostaining in the liver, whereas treatment with BSE (100 mg/kg) prevented these effects. Similarly, exposure to BSE (0.1–1 mg/mL) significantly reduced oxidative cell death induced by t-butyl hydroperoxide (t-BHP, 300 μM) and stabilized the mitochondrial membrane potential (∆ψ). BSE dose-dependently increased the activity of Nrf2, a potential transcriptional regulator of antioxidant genes, in HepG2 cells. Therefore, increased expression of its target genes, heme oxygenase-1 (HO-1), NADPH quinone oxidoreductase 1 (NQO1), and glutamate-cysteine ligase catalytic subunit (GCLC) was observed. Since GCLC is involved in the rate-limiting step of GSH synthesis, BSE increased the GSH level and decreased both cysteine dioxygenase (CDO) expression and taurine level. Because cysteine is a substrate for both taurine and GSH synthesis, a decrease in CDO expression would further contribute to increased cysteine availability for GSH synthesis. In conclusion, BSE protected the liver cells from oxidative stress by activating Nrf2 and increasing GSH synthesis.

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참고문헌 (42)

1. Yip W.W. Burt A.D. Alcoholic liver disease Semin. Diagn. Pathol. 2006 23 149 160 10.1053/j.semdp.2006.11.002 17355088

상세보기
2. Day C.P. Genes or environment to determine alcoholic liver disease and non-alcoholic fatty liver disease Liver Int. 2006 26 1021 1028 10.1111/j.1478-3231.2006.01323.x 17032401

상세보기
3. Wu D. Cederbaum A.I. Oxidative stress and alcoholic liver disease Semin. Liver Dis. 2009 29 141 154 10.1055/s-0029-1214370 19387914

상세보기
4. Nagata K. Suzuki H. Sakaguchi S. Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis J. Toxicol. Sci. 2007 32 453 468 10.2131/jts.32.453 18198478

상세보기
5. Bailey S.M. Mantena S.K. Millender-Swain T. Cakir Y. Jhala N.C. Chhieng D. Pinkerton K.E. Ballinger S.W. Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoe(-/-) mouse: Implications for a “multihit” hypothesis of fatty liver disease Free Radic. Biol. Med. 2009 46 928 938 10.1016/j.freeradbiomed.2009.01.003 19280709

상세보기
6. Albano E. Clot P. Morimoto M. Tomasi A. Ingelman-Sundberg M. French S.W. Role of cytochrome p4502e1-dependent formation of hydroxyethyl free radical in the development of liver damage in rats intragastrically fed with ethanol Hepatology 1996 23 155 163 10.1002/hep.510230121 8550035

상세보기
7. French S.W. Wong K. Jui L. Albano E. Hagbjork A.L. Ingelman-Sundberg M. Effect of ethanol on cytochrome p450 2e1 (cyp2e1), lipid peroxidation, and serum protein adduct formation in relation to liver pathology pathogenesis Exp. Mol. Pathol. 1993 58 61 75 10.1006/exmp.1993.1006 8454037

상세보기
8. Butura A. Nilsson K. Morgan K. Morgan T.R. French S.W. Johansson I. Schuppe-Koistinen I. Ingelman-Sundberg M. The impact of cyp2e1 on the development of alcoholic liver disease as studied in a transgenic mouse model J. Hepatol. 2009 50 572 583 10.1016/j.jhep.2008.10.020 19157621

상세보기
9. Abdelmegeed M.A. Banerjee A. Jang S. Yoo S.H. Yun J.W. Gonzalez F.J. Keshavarzian A. Song B.J. Cyp2e1 potentiates binge alcohol-induced gut leakiness, steatohepatitis, and apoptosis Free Radic. Biol. Med. 2013 65 1238 1245 10.1016/j.freeradbiomed.2013.09.009 24064383

상세보기
10. Mantena S.K. King A.L. Andringa K.K. Landar A. Darley-Usmar V. Bailey S.M. Novel interactions of mitochondria and reactive oxygen/nitrogen species in alcohol mediated liver disease World J. Gastroenterol. 2007 13 4967 4973 10.3748/wjg.v13.i37.4967 17854139

상세보기
11. Lieber C.S. Casini A. DeCarli L.M. Kim C.I. Lowe N. Sasaki R. Leo M.A. S-adenosyl-l-methionine attenuates alcohol-induced liver injury in the baboon Hepatology 1990 11 165 172 10.1002/hep.1840110203 2307395

상세보기
12. Song Z. Zhou Z. Chen T. Hill D. Kang J. Barve S. McClain C. S-adenosylmethionine (same) protects against acute alcohol induced hepatotoxicity in mice J. Nutr. Biochem. 2003 14 591 597 10.1016/S0955-2863(03)00116-5 14559110

상세보기
13. Ronis M.J. Butura A. Sampey B.P. Shankar K. Prior R.L. Korourian S. Albano E. Ingelman-Sundberg M. Petersen D.R. Badger T.M. Effects of n-acetylcysteine on ethanol-induced hepatotoxicity in rats fed via total enteral nutrition Free Radic. Biol. Med. 2005 39 619 630 10.1016/j.freeradbiomed.2005.04.011 16085180

상세보기
14. Ozaras R. Tahan V. Aydin S. Uzun H. Kaya S. Senturk H. N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat World J. Gastroenterol. 2003 9 125 128 10.3748/wjg.v9.i1.125 12508366

상세보기
15. Ma Q. Role of nrf2 in oxidative stress and toxicity Annu. Rev. Pharmacol. Toxicol. 2013 53 401 426 10.1146/annurev-pharmtox-011112-140320 23294312

상세보기
16. Wu K.C. Cui J.Y. Klaassen C.D. Effect of graded nrf2 activation on phase-i and -ii drug metabolizing enzymes and transporters in mouse liver PLoS ONE 2012 7 e39006 10.1371/journal.pone.0039006 22808024

상세보기
17. Kensler T.W. Wakabayashi N. Biswal S. Cell survival responses to environmental stresses via the keap1-nrf2-are pathway Annu. Rev. Pharmacol. Toxicol. 2007 47 89 116 10.1146/annurev.pharmtox.46.120604.141046 16968214

상세보기
18. Chan K. Han X.D. Kan Y.W. An important function of nrf2 in combating oxidative stress: Detoxification of acetaminophen Proc. Natl. Acad. Sci. USA 2001 98 4611 4616 10.1073/pnas.081082098 11287661

상세보기
19. Chowdhry S. Nazmy M.H. Meakin P.J. Dinkova-Kostova A.T. Walsh S.V. Tsujita T. Dillon J.F. Ashford M.L. Hayes J.D. Loss of nrf2 markedly exacerbates nonalcoholic steatohepatitis Free Radic. Biol. Med. 2010 48 357 371 10.1016/j.freeradbiomed.2009.11.007 19914374

상세보기
20. Lu C. Xu W. Zhang F. Shao J. Zheng S. Nrf2 knockdown disrupts the protective effect of curcumin on alcohol-induced hepatocyte necroptosis Mol. Pharm. 2016 13 4043 4053 10.1021/acs.molpharmaceut.6b00562 27764939

상세보기
21. Reisman S.A. Buckley D.B. Tanaka Y. Klaassen C.D. Cddo-im protects from acetaminophen hepatotoxicity through induction of nrf2-dependent genes Toxicol. Appl. Pharmacol. 2009 236 109 114 10.1016/j.taap.2008.12.024 19371629

상세보기
22. Okawa H. Motohashi H. Kobayashi A. Aburatani H. Kensler T.W. Yamamoto M. Hepatocyte-specific deletion of the keap1 gene activates nrf2 and confers potent resistance against acute drug toxicity Biochem. Biophys. Res. Commun. 2006 339 79 88 10.1016/j.bbrc.2005.10.185 16293230

상세보기
23. Lee Y.H. Kim J.H. Kim S.H. Oh J.Y. Seo W.D. Kim K.M. Jung J.C. Jung Y.S. Barley sprouts extract attenuates alcoholic fatty liver injury in mice by reducing inflammatory response Nutrients 2016 8 440 10.3390/nu8070440 27455313

상세보기
24. Volpi N. Tarugi P. Improvement in the high-performance liquid chromatography malondialdehyde level determination in normal human plasma J. Chromatogr. B Biomed. Sci. Appl. 1998 713 433 437 10.1016/S0378-4347(98)00195-9 9746260

상세보기
25. Jung Y.S. Kim S.J. Kwon D.Y. Kim Y.C. Comparison of the effects of buthioninesulfoximine and phorone on the metabolism of sulfur-containing amino acids in rat liver Biochem. Biophys. Res. Commun. 2008 368 913 918 10.1016/j.bbrc.2008.02.005 18275846

상세보기
26. Ide T. Simple high-performance liquid chromatographic method for assaying cysteinesulfinic acid decarboxylase activity in rat tissue J. Chromatogr. B Biomed. Sci. Appl. 1997 694 325 332 10.1016/S0378-4347(97)00128-X 9252046

상세보기
27. Nolin T.D. McMenamin M.E. Himmelfarb J. Simultaneous determination of total homocysteine, cysteine, cysteinylglycine, and glutathione in human plasma by high-performance liquid chromatography: Application to studies of oxidative stress J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 2007 852 554 561 10.1016/j.jchromb.2007.02.024 17336168

상세보기
28. Castillo T. Koop D.R. Kamimura S. Triadafilopoulos G. Tsukamoto H. Role of cytochrome P-450 2E1 in ethanol-, carbon tetrachloride- and iron-dependent microsomal lipid peroxidation Hepatology 1992 16 992 996 10.1002/hep.1840160423 1398507

상세보기
29. Muller A. Sies H. Role of alcohol dehydrogenase activity and the acetaldehyde in ethanol- induced ethane and pentane production by isolated perfused rat liver Biochem. J. 1982 206 153 156 10.1042/bj2060153 6751324

상세보기
30. Kera Y. Komura S. Ohbora Y. Kiriyama T. Inoue K. Ethanol induced changes in lipid peroxidation and nonprotein sulfhydryl content. Different sensitivities in rat liver and kidney Res. Commun. Chem. Pathol. Pharmacol. 1985 47 203 209 3992007
31. Videla L.A. Fernandez V. Ugarte G. Valenzuela A. Effect of acute ethanol intoxication on the content of reduced glutathione of the liver in relation to its lipoperoxidative capacity in the rat FEBS Lett. 1980 111 6 10 10.1016/0014-5793(80)80749-6 7358166

상세보기
32. Vina J. Estrela J.M. Guerri C. Romero F.J. Effect of ethanol on glutathione concentration in isolated hepatocytes Biochem. J. 1980 188 549 552 10.1042/bj1880549 6994718

상세보기
33. Kono H. Rusyn I. Bradford B.U. Connor H.D. Mason R.P. Thurman R.G. Allopurinol prevents early alcohol-induced liver injury in rats J. Pharmacol. Exp. Ther. 2000 293 296 303 10734182

상세보기
34. Kono H. Arteel G.E. Rusyn I. Sies H. Thurman R.G. Ebselen prevents early alcohol-induced liver injury in rats Free Radic. Biol. Med. 2001 30 403 411 10.1016/S0891-5849(00)00490-1 11182296

상세보기
35. Kono H. Rusyn I. Uesugi T. Yamashina S. Connor H.D. Dikalova A. Mason R.P. Thurman R.G. Diphenyleneiodonium sulfate, an nadph oxidase inhibitor, prevents early alcohol-induced liver injury in the rat Am. J. Physiol. Gastrointest. Liver Physiol. 2001 280 G1005 G1012 11292610
36. McKim S.E. Konno A. Gabele E. Uesugi T. Froh M. Sies H. Thurman R.G. Arteel G.E. Cocoa extract protects against early alcohol-induced liver injury in the rat Arch. Biochem. Biophys. 2002 406 40 46 10.1016/S0003-9861(02)00425-3 12234488

상세보기
37. Lamle J. Marhenke S. Borlak J. von Wasielewski R. Eriksson C.J. Geffers R. Manns M.P. Yamamoto M. Vogel A. Nuclear factor-eythroid 2-related factor 2 prevents alcohol-induced fulminant liver injury Gastroenterology 2008 134 1159 1168 10.1053/j.gastro.2008.01.011 18395094

상세보기
38. Wu K.C. Liu J. Klaassen C.D. Role of nrf2 in preventing ethanol-induced oxidative stress and lipid accumulation Toxicol. Appl. Pharmacol. 2012 262 321 329 10.1016/j.taap.2012.05.010 22627062

상세보기
39. Lu C. Zhang F. Xu W. Wu X. Lian N. Jin H. Chen Q. Chen L. Shao J. Wu L. Curcumin attenuates ethanol-induced hepatic steatosis through modulating nrf2/fxr signaling in hepatocytes IUBMB Life 2015 67 645 658 10.1002/iub.1409 26305715

상세보기
40. Meister A. Anderson M.E. Glutathione Annu. Rev. Biochem. 1983 52 711 760 10.1146/annurev.bi.52.070183.003431 6137189

상세보기
41. Kwon Y.H. Stipanuk M.H. Cysteine regulates expression of cysteine dioxygenase and gamma-glutamylcysteine synthetase in cultured rat hepatocytes Am. J. Physiol. Endocrinol. Metab. 2001 280 E804 E815 11287364
42. Stipanuk M.H. Dominy J.E. Lee J.I. Coloso R.M. Mammalian cysteine metabolism: New insights into regulation of cysteine metabolism J. Nutr. 2006 136 1652S 1659S 16702335

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Antioxidant Effect of Barley Sprout Extract via Enhancement of Nuclear Factor-Erythroid 2 Related Factor 2 Activity and Glutathione Synthesis 원문보기

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Antioxidant Effect of Barley Sprout Extract via Enhancement of Nuclear Factor-Erythroid 2 Related Factor 2 Activity and Glutathione Synthesis 원문보기

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