[논문]Glehnia littoralis Root Extract Inhibits Fat Accumulation in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice by Downregulating Adipogenic Gene Expression

Hong, Heeok; dela Cruz, Joseph F.; Kim, Won Seob; Yoo, Kiyeol; Hwang, Seong Gu

doi:10.1155/2018/1243049

Glehnia littoralis Root Extract Inhibits Fat Accumulation in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice by Downregulating Adipogenic Gene Expression 원문보기

Evidence-based Complementary and Alternative Medicine : eCAM, v.2018, 2018년, pp.1243049 -

Hong, Heeok (Department of Medical Science, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea) , dela Cruz, Joseph F. (Department of Animal Life and Environmental Science, Hankyong National University, Anseong 17579, Republic of Korea) , Kim, Won Seob (Department of Animal Science and Technology, Konkuk University, Seoul 05029, Republic of Korea) , Yoo, Kiyeol (Department of Biological Sciences, Dankook University, Cheonan 31116, Republic of Korea) , Hwang, Seong Gu (Department of Animal Life and Environmental Science, Hankyong National University, Anseong 17579, Republic of Korea)

Abstract ▼ AI-Helper

Glehnia littoralis has been reported to have several pharmacological properties but no reports describing the antiadipogenic effect of this plant have been published. This study was conducted to investigate the effects of Glehnia littoralis root hot water extract (GLE) and its underlying mechanism on 3T3-L1 cell adipogenesis and in high-fat diet- (HFD-) induced obese mice. We measured intracellular lipid accumulation using oil red O staining in vitro. For in vivo study, twenty-eight C57BL/6J male mice were randomly divided into four groups, Control, HFD, HFD + 1% GLE, and HFD + 5% GLE, which was performed for eight weeks. We determined the expression levels of the adipogenesis-related proteins by RT-PCR and western blotting in HFD-induced obese mice. The GLE dose-dependently inhibited 3T3-L1 adipocyte differentiation and intracellular lipid accumulation in differentiated adipocytes. Further, body weight gain and fat accumulation were significantly lower in the GLE-treated HFD mice than in the untreated HFD mice. GLE treatment suppressed the expression of adipogenic genes such as peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer-binding protein (C/EBP) α, fatty acid synthase (aP2), and fatty acid synthase (FAS). These results suggest that the GLE inhibits adipocyte differentiation and intracellular lipid accumulation by downregulating the adipogenic gene expression both in vitro and in vivo.

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Glehnia littoralis Root Extract Inhibits Fat Accumulation in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice by Downregulating Adipogenic Gene Expression 원문보기

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