[논문]Anti-rheumatoid arthritis effects of flavonoids from Daphne genkwa

Sun, Yue-wen; Bao, Yarigui; Yu, Hui; Chen, Qiu-jing; Lu, Fang; Zhai, Shuo; Zhang, Chun-feng; Li, Fei; Wang, Chong-zhi; Yuan, Chun-su

doi:10.1016/j.intimp.2020.106384

Anti-rheumatoid arthritis effects of flavonoids from Daphne genkwa

International immunopharmacology, v.83, 2020년, pp.106384 -

Sun, Yue-wen (School of Traditional Chinese Pharmacy, China Pharmaceutical University) , Bao, Yarigui (School of Traditional Chinese Pharmacy, China Pharmaceutical University) , Yu, Hui (Shandong Drug and Food Vocational College) , Chen, Qiu-jing (School of Traditional Chinese Pharmacy, China Pharmaceutical University) , Lu, Fang (School of Traditional Chinese Pharmacy, China Pharmaceutical University) , Zhai, Shuo (School of Traditional Chinese Pharmacy, China Pharmaceutical University) , Zhang, Chun-feng (School of Traditional Chinese Pharmacy, China Pharmaceutical University) , Li, Fei (School of Pharmacy, Xinjiang Medical University) , Wang, Chong-zhi (Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago) , Yuan, Chun-su (Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago USA<)

Abstract ▼ AI-Helper

Abstract Objective This study aims to select the most effective anti-Rheumatoid Arthritis (RA) component of flavonoids from Daphne genkwa Sieb. et Zucc. by anti-inflammatory and immunomodulatory effects in vitro, and to elucidate the mechanism. Methods The anti-inflammatory and immunomodulatory effects of total flavonoids (TF) and four flavonoid components (genkwanin, hydroxygenkwanin, luteolin and apigenin) were determined by pharmacological approach in LPS-induced RAW 264.7 macrophages and ConA-induced T lymphocytes. Principal component analysis (PCA) was used to obtain the optimal anti-RA component in vitro. Western blot and real-time quantitative PCR (q-PCR) were used to explore the mechanisms. Finally, the in vitro anti-RA effect was verified by human rheumatoid arthritis fibroblast-like synoviocytes (FLSs). Results TF and four flavonoids significantly reduced the expressions of NO, iNOS, TNF-α, IL-6, IFN-γ and IL-2. PCA showed that genkwanin was the most effective anti-RA component in vitro. Genkwanin inhibited nuclear factor-κB (NF-κB) pathway by decreasing the phosphorylation levels of IKK, IκB and NF-κB, and down-regulated the expressions of iNOS, COX-2 and IL-6 mRNA. Genkwanin also inhibited the abnormal proliferation of FLSs and down-regulated the secretions of NO and IL-6. Conclusion The most effective anti-RA component was genkwanin. Genkwanin exerts anti-RA effect through down-regulating the activation of NF-κB pathway and mRNA expressions of inflammatory mediators, and also by inhibiting the abnormal proliferation of FLSs and its NO and IL-6 secretion levels. Highlights Flavonoids from Daphne genkwa had anti-inflammatory activity and immunomodulatory activity. Genkwanin was the most effective anti-RA component of flavonoids from Daphne genkwa. Genkwanin inhabited NF-κB pathway. Genkwanin down-regulated the expression of iNOS, COX-2 and IL-6 mRNA. Genkwanin inhibited the abnormal proliferation of FLSs.

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표제어: PCR

동의어: Packet Collision Rate

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Anti-rheumatoid arthritis effects of flavonoids from Daphne genkwa

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