초록 ▼

The susceptibility of bacteria, particularly those derived from body fluids, to antimicrobial agents is determined in terms of an ATP index measured by culturing a bacterium in a growth medium, assaying the amount of ATP in a sample of the cultured bacterium by measuring the amount of luminescent li

The susceptibility of bacteria, particularly those derived from body fluids, to antimicrobial agents is determined in terms of an ATP index measured by culturing a bacterium in a growth medium, assaying the amount of ATP in a sample of the cultured bacterium by measuring the amount of luminescent light emitted when the bacterial ATP is reacted with a luciferase-luciferin mixture, subjecting the sample of the cultured bacterium to an antibiotic agent and assaying the amount of bacterial adenosine triphosphate after treatment with the antibiotic by measuring the luminescent light resulting from the reaction, whereby the ATP index is determined from the values obtained from the assay procedures.

대표청구항 ▼

A method for determining the sensitivity of bacteria to antimicrobial agents by measurement of the ATP index indicating sensitivity, which comprises: culturing bacterium in a growth medium; developing a uniform inoculum; preincubating the inoculum for a period sufficient to initiate growth; aliquoti

A method for determining the sensitivity of bacteria to antimicrobial agents by measurement of the ATP index indicating sensitivity, which comprises: culturing bacterium in a growth medium; developing a uniform inoculum; preincubating the inoculum for a period sufficient to initiate growth; aliquoting at least three test portions, at least one test portion having an antibiotic added and at least two test portions without antibiotic added; assaying the amount of bacterial adenosine triphosphate in the test portions, one assay on a test portion without antibiotic being made prior to any incubation, the other assays being made after incubation; determining the ATP index to indicate sensitivity of the bacterium to the antibiotic; the ATP index being determined according to the formula: ATP index =Bt -Ao/At -Ao, where Bt represents a light reading for a test portion treated with antibiotic and allowed to incubate for a time t; At represents a light reading for a test portion not treated with antibiotic and allowed to incubate for a time t; and Ao represents a light reading for a test portion not treated with antibiotic and which is not allowed to incubate, at time zero.