Immobilized ribonuclease and alkaline phosphatase
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07G-007/02
C12B-001/00
출원번호
US-0678459
(1976-04-19)
우선권정보
FR-0012667 (1975-04-23)
발명자
/ 주소
Thang Minh-Nguy (Bagneux FR) Guissani born Trachtenberg Annie (Fresnes FR)
출원인 / 주소
Choay S. A. (Paris FR 03)
인용정보
피인용 횟수 :
6인용 특허 :
0
초록▼
An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T1, T2, U2 and an alkaline phosphatease. Fr
An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T1, T2, U2 and an alkaline phosphatease. Free activated groups of the matrix after binding of the enzymes, are neutralized by a free amino organic base. The support is selected from among non-denaturing supports effecting the irreversible physical adsorption of the enzymes, such as supports of glass or quartz beads, highly cross-linked gels of the agarose or cellulose type. Polymers AnUOH, AnCOH, AnGOH and/or oligonucleotides U, C, A or G, of predetermined lengths, can be obtained by fractionating the polyribonucleotides by incubation with the insoluble solid matrix.
대표청구항▼
An insoluble solid matrix carrying simultaneously more than one different enzymatic function, consisting essentially of: an activated solid insoluble matrix support, non-denaturing for the enzymes to be attached thereto; a nuclease, selected from the group consisting of ribonucleases A, T1, T2 and U
An insoluble solid matrix carrying simultaneously more than one different enzymatic function, consisting essentially of: an activated solid insoluble matrix support, non-denaturing for the enzymes to be attached thereto; a nuclease, selected from the group consisting of ribonucleases A, T1, T2 and U2; and an alkaline phosphatase, said nuclease and said alkaline phosphatase being irreversibly bound to said matrix support, wherein the enzyme-bound matrix has no non-neutralized free activated groups thereon. Process for obtaining polymers AnUOH, AnCOH, AnGOH and/or oligonucleotides U, C, A or G, of predetermined lengths, said process comprising: fractionating a polyribonucleotide by incubation with the insoluble solid matrix carrying simultaneously more than one different enzymatic function in accordance with claim 1.
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이 특허를 인용한 특허 (6)
Hartmeier Winfried (Ingelheim am Rhein DEX), Glucose oxidation with immobilized glucose oxidase-catalase.
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