초록 ▼

Method and apparatus for the detection of antibodies and antigens based upon the effect that any arbitrary antigen will adsorb onto a substrate in a monomolecular layer only, but that a corresponding specific antibody for such arbitrary antigen will bond thereto to form a bimolecular layer on the su

Method and apparatus for the detection of antibodies and antigens based upon the effect that any arbitrary antigen will adsorb onto a substrate in a monomolecular layer only, but that a corresponding specific antibody for such arbitrary antigen will bond thereto to form a bimolecular layer on the substrate. A first layer of antigen is adsorbed onto a substrate and the coated substrate is then exposed to a solution suspected of containing the specific antibody of interest. The substrate is then examined to determine whether a monomolecular or bimolecular layer is adhering thereon. Optical, including visual, electrical, and chemical means for examining the coated substrate are disclosed. The method can be reversed by replacing the antigen in each step above with its specifically reacting antibody and by replacing the antibody with its specific antigen.

대표청구항 ▼

A diagnostic method for determining the presence or absence of a specific antibody or antigen in a biological sample comprising the steps of: depositing a metal onto a substrate material; contacting said substrate material with an aqueous medium containing either a corresponding specifically reactin

A diagnostic method for determining the presence or absence of a specific antibody or antigen in a biological sample comprising the steps of: depositing a metal onto a substrate material; contacting said substrate material with an aqueous medium containing either a corresponding specifically reacting antigen to said specific antibody or a corresponding specifically reacting antibody to said specific antigen, to coat all or part of said substrate material with a monomolecular layer of said specifically reacting antigen or antibody; immersing said coated substrate material in said sample; and examining said coated substrate material to determine whether said substrate has a bimolecular or monomolecular layer adhering thereto.