초록 ▼

Improved cell culture microcarriers, and methods for their production and use, are disclosed herein. These improved microcarriers have positive charge capacities adjusted and/or controlled within a range suitable for good cell growth. One method for producing such improved microcarriers is by treati

Improved cell culture microcarriers, and methods for their production and use, are disclosed herein. These improved microcarriers have positive charge capacities adjusted and/or controlled within a range suitable for good cell growth. One method for producing such improved microcarriers is by treating beads formed from polymers containing pendant hydroxy groups, such as dextran beads, with an aqueous solution of an alkaline material and a chloro- or bromo-substituted tertiary amine under precisely controlled conditions to produce the desired exchange capacity. The resultant positively charged microcarriers have been used in microcarrier cultures to produce outstanding growth of anchorage-dependent cells. Such cells can be harvested, or used for the production of viruses, vaccines, hormones, interferon or other cellular growth by-products.

대표청구항 ▼

In a method of growing anchorage-dependent cells in microcarrier culture, the improvement of employing microcarriers having an amount of positively charged chemical moieties thereon adjusted to provide an exchange capacity which allows good growth of said cells, said exchange capacity being within t

In a method of growing anchorage-dependent cells in microcarrier culture, the improvement of employing microcarriers having an amount of positively charged chemical moieties thereon adjusted to provide an exchange capacity which allows good growth of said cells, said exchange capacity being within the range of between about 0.1 and about 4.5 meq/gram of dry, untreated microcarriers.