초록 ▼

Method for making a collagen sponge including the following steps. Native collagen form of fibrillar protein is comminuted and homogenized in an acid environment; noncollagenous material is removed and residual collagen is mixed with water the pH of the resulting slurry is adjusted to 4.5 to 5.0 by

Method for making a collagen sponge including the following steps. Native collagen form of fibrillar protein is comminuted and homogenized in an acid environment; noncollagenous material is removed and residual collagen is mixed with water the pH of the resulting slurry is adjusted to 4.5 to 5.0 by the addition of acetic acid; gluteraldehyde is then added and the slurry poured into molds and frozen at approximately -10°centigrade for approximately 20 hours. The frozen mass is then thawed, washed, and immersed in a wash of pH 8 to 9 for approximately 2 hours at 20°centigrade. Sufficient reducing agent is added to the wash to create excess reducing equivalents. The sponge is then immersed in a buffer solution of pH 4 to 5 for a time sufficient to equilibrate to uniform pH. The sponge formed by the above method is then moistended and self-administered in the upper vault of the vagina proximal to the cervix. The sponge is then retained for a desired period, usually several days to one month.

대표청구항 ▼

In a method of making a collagenous product wherein comminuted collagen is suspended in water and formed into a homogeneous slurry, the improvement comprising: in the folowing sequence (a) adjusting the pH of said slurry to from pH 3.5 to pH 6.5; (b) adding from 0.5% to 1% of an aldehyde based on t

In a method of making a collagenous product wherein comminuted collagen is suspended in water and formed into a homogeneous slurry, the improvement comprising: in the folowing sequence (a) adjusting the pH of said slurry to from pH 3.5 to pH 6.5; (b) adding from 0.5% to 1% of an aldehyde based on the total volume of said slurry; (c) freezing said slurry at a temperature of from 0°centigrade to -20°centigrade; (d) thawing said slurry to form collagenous sponge; and (e) immersing said sponge in a reducing wash having a pH of from 8 to 9 containing sufficient reducing agent to create excess reducing equivalents for a time, and at a temperature sufficient to reduce the existing double bond Schiff base to a single bond.