초록 ▼

A method and solid phase matrix is disclosed which has special utility for use in conjunction with automatic pipetting apparatus for conducting reactions at a liquid-solid interface. A reaction component is fixed on the surface of the solid phase matrix which is wetted by a liquid phase containing a

A method and solid phase matrix is disclosed which has special utility for use in conjunction with automatic pipetting apparatus for conducting reactions at a liquid-solid interface. A reaction component is fixed on the surface of the solid phase matrix which is wetted by a liquid phase containing a freely diffusing, mobile component. The matrix is comprised of a support surface to which are attached a plurality of inwardly projecting fins. The innermost extension of the fins defines a centrally located well into which an automatic pipetting apparatus may be inserted. The method may be used for qualitative or quantitative determinations of the mobile component contained in a liquid which wets the solid phase matrix.

대표청구항 ▼

A process for detecting a mobile component contained in a liquid sample by reacting the mobile component with a fixed reaction component disposed on the surface of a solid phase reaction matrix comprising: (a) adding a volume of a liquid sample having a mobile component to a water-insoluble solid-ph

A process for detecting a mobile component contained in a liquid sample by reacting the mobile component with a fixed reaction component disposed on the surface of a solid phase reaction matrix comprising: (a) adding a volume of a liquid sample having a mobile component to a water-insoluble solid-phase reaction matrix having an elongated annular support surface closed at one end and open at the other end thereof, the annular surface defining a first region, into which a volume of liquid may be added, and a plurality of inwardly extending projections attached to the inner wall of the support surface, the spacing between said inwardly extending projections being sufficient to permit removal of the liquid sample by decantation or aspiration, the plurality of inwardly extending projections being so arranged that they define a second generally cylindrical region centrally disposed within the first region into which may be inserted a pipetting means for dispensing and removing the liquid sample, the second region being substantially smaller than the first region, the interior of the annular surface and each of the projecting surfaces of said matrix having a reaction component immobilized thereon, said reaction component selected from the group consisting of antigens, antibodies, enzymes and substrates and being capable of reacting specifically with said mobile component at a rate or to an extent measurable as a function of the concentration of the molecules of the mobile component, the volume of liquid sample being added by insertion of the pipetting means into the second region to wet the solid-phase matrix; (b) contacting the liquid sample with the inwardly extending and inner annular surfaces of the reaction matrix; (c) maintaining the liquid sample in contact with the solid-phase matrix surfaces for a given period of time to react the fixed component with any mobile component present in the liquid sample; (d) removing the liquid sample from the contact with the solid-phase reaction matrix by decantation or insertion of the pipetting means into the second region; and (e) detecting if any of the mobile component has reacted with the fixed reaction component disposed on the solid-phase reaction matrix.