초록

Cellulase, an enzyme useful in the saccharification of cellulose can be recycled by a process of selective adsorption on cellulose containing materials and readmitted to the reaction mixture in a batch, semi-continuous, or continuous simultaneous saccharification-fermentation process.

대표청구항 ▼

A method of recycling the endoglucanase (E.C.3.2.1.4) and cellobiohydrolase (E.C.3.2.1.91) portions of activity of cellulase enzyme which had been used as the cellulose hydrolyzing enzyme in a simultaneous saccharification-fermentation reaction of cellulose-to-ethanol by: (1) separating the liquid f

A method of recycling the endoglucanase (E.C.3.2.1.4) and cellobiohydrolase (E.C.3.2.1.91) portions of activity of cellulase enzyme which had been used as the cellulose hydrolyzing enzyme in a simultaneous saccharification-fermentation reaction of cellulose-to-ethanol by: (1) separating the liquid fraction from the reaction mixture; (2) contacting this enzyme and alcohol-containing liquid fraction with a cellulose-containing solid which is useful for the simultaneous saccharification-fermentation reaction at between about pH 5 to about pH 8 and at above an ionic strength of 0.05 M to adsorb the enzymes thereupon; (3) separating the solid fraction containing the adsorbed enzymes; and (4) using the solid enzyme-cellulose combination as a portion of the feed to a further simultaneous saccharification-fermentation reaction.