초록 ▼

Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and

Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding.

대표청구항 ▼

A method for the separation of a select population of cells, bacteria, or viruses from a mixed population thereof, in which microspheres containing magnetic particles are coated with a layer of antibodies which selectively bind to the select population, the coated microspheres are contacted with sai

A method for the separation of a select population of cells, bacteria, or viruses from a mixed population thereof, in which microspheres containing magnetic particles are coated with a layer of antibodies which selectively bind to the select population, the coated microspheres are contacted with said mixed population so that said microspheres are bound to the select population, and said bound select population is magentically separated from the rest of said mixed population, wherein the improvement comprises: prior to coating said microspheres with antibodies modifying the surfaces of said microspheres to provide staphylococcal Protein A distributed thereover in adherent relation to said microspheres, then contacting said microspheres with antibodies which bind to Protein A and which also bind selectively to said select population, whereby said antibodies are arranged in oriented attachment on the surfaces of said microspheres with their Fab arms extending outwardly, and thereafter carrying out the rest of the steps of said method.