초록 ▼

A specific binding assay method and reagent means for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium which employs a fluorescent substance, i.e., a fluorescer, as a label. The improvement comprises measuring the fluorescer-label by chemic

A specific binding assay method and reagent means for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium which employs a fluorescent substance, i.e., a fluorescer, as a label. The improvement comprises measuring the fluorescer-label by chemically exciting the label and measuring the resulting light emitted thereby. Chemical excitation of the label is preferably accomplished by exposure to a substance, such as a high energy intermediate produced by the reaction between hydrogen peroxide and highly reactive materials such as oxalyl chloride, oxamides and bis-oxalate esters. The assay may follow conventional homogeneous and heterogeneous formats. The improved assay is more convenient than conventional fluorescent binding assays by obviating the need for photogenic excitation of the fluorescent label.

대표청구항 ▼

In a specific binding assay method for determining a ligand in or the ligand binding capacity of a liquid medium, wherein said liquid medium is combined with reagent means including a labeled conjugate comprising a binding component incorporated with a fluorescent label, said combination forming a b

In a specific binding assay method for determining a ligand in or the ligand binding capacity of a liquid medium, wherein said liquid medium is combined with reagent means including a labeled conjugate comprising a binding component incorporated with a fluorescent label, said combination forming a binding reaction system having a bound-species and a free-species of the labeled conjugate, the amount of said fluorescent label resulting in either of said bound-species or said free-species being a function of the presence or amount of said ligand or ligand binding capacity in said liquid medium, and wherein said fluorescent label is measured, the improvement which comprises measuring said fluorescent label by chemically exciting said label to cause the same to emit light, and then measuring the light emitted by the excited fluorescent label. A test kit for use in determining a ligand in a liquid medium by a specific binding assay method, comprising, in a packaged combination, one or more containers holding (1) said ligand, or a binding analog thereof, incorporated with a fluorescent label, (2) a binding partner for said ligand, and (3) chemical reagents capable of reacting to produce a high energy intermediate which excites said fluorescent label to cause same to emit light.