초록 ▼

A test device for assaying liquid samples for the presence of a predetermined reagent is described. The device comprises telescoping top and bottom members defining a liquid reservoir therebetween, resilient means for biasing the members in the open position, one or more test wells in the top member

A test device for assaying liquid samples for the presence of a predetermined reagent is described. The device comprises telescoping top and bottom members defining a liquid reservoir therebetween, resilient means for biasing the members in the open position, one or more test wells in the top member with bottom openings covered with microporous membranes, a co-reactant immobilized on the membrane surfaces, and sorbent means between the members spaced from the membrane(s) in the open position but in contact therewith in the closed position. Liquids are passed through the membrane(s) into the sorbent means by depressing the members to the closed position.

대표청구항 ▼

A test device for assaying a test liquid for the presence of a predetermined reactant by incubation with a coreactant therefor to form and wash a detectable reaction product, said device comprising: top and bottom members disposed for relative movement toward and away from each other to define an op

A test device for assaying a test liquid for the presence of a predetermined reactant by incubation with a coreactant therefor to form and wash a detectable reaction product, said device comprising: top and bottom members disposed for relative movement toward and away from each other to define an open position and a closed position, means for biasing said members in the open position, at least one well for test liquid in the top member, said well having an open bottom, a microporous membrane having substantially uniform pores having between about 25 nanometers and 25 micrometers sealed liquid-tight to the periphery of and extending substantially horizontally across the open bottom of the well, said well or membrane containing or constituting said coreactant, and solid sorbent material carried by said bottom member, the major portion of said material being wettable by said liquid, the upper surface of said sorbent material being disposed for contact with the bottom of said membrane when the members are in the closed position but being removed therefrom in said open position, whereby liquid is retained in said well when the members are in the open position but flows through the membrane into the sorbent material in the closed position. The method of assaying for the presence of a predetermined reactant in a test liquid which comprises: providing a test device according to any one of claims 1, 2, 3 or 4, the top and bottom members thereof being in the biased open position, placing test liquid in said well and incubating for a time sufficient to form said reaction product, moving said members toward each other from the open to the closed position and allowing the test liquid to flow through the membrane into the sorbent material, moving said members away from each other to the open position, adding a washing liquid to said well, moving said members toward each other from the open to the closed position and allowing the washing liquid to flow through the membrane into the sorbent material, and determining the presence or absence of said reaction product.