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A chromatography column adapted for high pressure chromatography is characterized by a tubular membrane separating a glass tube from the pressurized area between the glass tube and a pressure tube adapted to receive a pressurizing fluid. Changing of the glass tube is facilitated by annular seals at

A chromatography column adapted for high pressure chromatography is characterized by a tubular membrane separating a glass tube from the pressurized area between the glass tube and a pressure tube adapted to receive a pressurizing fluid. Changing of the glass tube is facilitated by annular seals at each end of the glass tube against which an adjustable threaded plug, fitted in one of a pair of screw caps fitted on each end of the pressure tube, exerts sealing pressure.

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In a chromatography column adapted for use in high pressure liquid chromatography comprising a glass tube open at both ends and positioned axially within a pressure tube having an internal diameter larger than the external diameter of the glass tube so as to provide a circumferential intermediate sp

In a chromatography column adapted for use in high pressure liquid chromatography comprising a glass tube open at both ends and positioned axially within a pressure tube having an internal diameter larger than the external diameter of the glass tube so as to provide a circumferential intermediate space therebetween and having an aperature in the wall thereof to provide communication between the intermediate space and a source of a pressurizing fluid when the column is connected therewith; a pair of removable screw caps threadably mounted on respective ends of the pressure tube, each having an aperature therein, communicating with respective ends of the glass tube and providing an inlet and outlet, respectively, for chromatography eluent; and sealing means for sealing the open ends of the glass column from the intermediate space, the improvement wherein the sealing means comprises a flexible tubular shaped membrane positioned in the intermediate space and extending the length of the glass tube so as to provide a sleeve therefor which separates the glass tube from the pressurizing fluid.