초록 ▼

Biological fluids, such as serum, are analyzed for the presence, nature and/or amount of antibodies, antigens and antibody:antigen complexes therein using as a reagent, insolubilized rheumatoid factor or insolubilized Clq. These reagents are themselves novel. They bind to antibody:antigen complexes,

Biological fluids, such as serum, are analyzed for the presence, nature and/or amount of antibodies, antigens and antibody:antigen complexes therein using as a reagent, insolubilized rheumatoid factor or insolubilized Clq. These reagents are themselves novel. They bind to antibody:antigen complexes, but not to free antibodies or antigens, and complexes can thus be removed from mixtures thereof with other materials, such as antibodies and antigens.

대표청구항 ▼

A reagent useful in biological analysis which comprises RF or Clq covalently bonded to a solid phase substrate which is insoluble in aqueous fluids. A method according to claim 8 wherein the substrate reacts with Ab:Ag complex by passing the fluid sample through the hollow tube. A method of competit

A reagent useful in biological analysis which comprises RF or Clq covalently bonded to a solid phase substrate which is insoluble in aqueous fluids. A method according to claim 8 wherein the substrate reacts with Ab:Ag complex by passing the fluid sample through the hollow tube. A method of competitive binding assay by which the presence, in a sample, of a particular antibody, antigen or antibody -antigen complex may be detected, and the amount thereof determined, which comprises the steps of: (a) adding to the sample to form a mixture a quantity of the specific antigen or antibody for the antibody or antigen, respectively, to be determined, or a quantity of the antibody -antigen complex to be determined, which said quantity carries an identifying label; (b) contacting the mixture with a reagent substance selected from the group consisting of RF and Clq, said substance being adsorbed onto a solid phase substrate which is insoluble in aqueous fluids; (c) separating the reagent substance from the mixture; and (d) measuring by the identifying label the amount of labelled antibody, antigen, or antibody -antigen complex remaining in the mixture or separated with the reagent, thereby determining the presence and amount of antibody, antigen or antibody -antigen complex in the original sample.