초록 ▼

Methods and apparatuses are featured for analyzing whole blood samples by diffusion techniques in porous media. A fluid component of the whole blood is diffused into a thin-film porous medium of a given predetermined volume. Hematocrit dependent errors in diffusion time do not pose a problem in obta

Methods and apparatuses are featured for analyzing whole blood samples by diffusion techniques in porous media. A fluid component of the whole blood is diffused into a thin-film porous medium of a given predetermined volume. Hematocrit dependent errors in diffusion time do not pose a problem in obtaining a precise aliquot of the fluid component of the whole blood due to rapid diffusion and saturation of the fluid component into the porous medium. In another embodiment, diffusion switches or valves allow for precise aliquots in porous media by control of the diffusion and/or reaction of sample analyte with reagents.

대표청구항 ▼

A method of performing a reaction with an analyte of a sample, comprising the steps of: (a) diffusing said analyte into a first porous medium; (b) contacting said first porous medium with a second porous medium to allow diffusion of said analyte into said second porous medium, said second porous med

A method of performing a reaction with an analyte of a sample, comprising the steps of: (a) diffusing said analyte into a first porous medium; (b) contacting said first porous medium with a second porous medium to allow diffusion of said analyte into said second porous medium, said second porous medium having at least one first reagent for reaction with said analyte; and (c) contacting said second porous medium with a third porous medium containing at least one second reagent, said contacting of at least two of said porous media comprising the displacement of a liquid from between said two media.