초록 ▼

Apparatus for harvesting material from micro-culture plates comprises either a harvester plate or, in combination a harvester plate and a micro-culture plate, the harvester plate having a plurality of wells recessed into one surface thereof, these wells conforming in size, number and arrangement to

Apparatus for harvesting material from micro-culture plates comprises either a harvester plate or, in combination a harvester plate and a micro-culture plate, the harvester plate having a plurality of wells recessed into one surface thereof, these wells conforming in size, number and arrangement to the wells of the micro-culture plate, and the wells of the harvester plate being adapted to hold filter elements for absorbing liquid from corresponding wells of the culture plate when the wells of the culture plate, with the culture plate in an upside-down orientation, are contacted with corresponding wells of the harvester plate, and in which the harvester plate wells have fluid outlets at their bases. Also a new micro-culture plate comprises a plurality of wells recessed into a surface thereof each well comprising a fluid inlet passageway through the base thereof. Additionally a new method of harvesting material from a micro-culture plate comprises harvesting material from the wells of the micro-culture plate when the plate is maintained in an upside-down orientation, the material being transferred directly to absorbent elements such as filter paper discs.

대표청구항 ▼

Apparatus for harvesting material from microculture plates comprising, in combination, a micro-culture plate and a harvester plate, the harvester plate having a plurality of wells recessed into one surface thereof, these wells conforming in size, number, and arrangement to the wells of the micro-cul

Apparatus for harvesting material from microculture plates comprising, in combination, a micro-culture plate and a harvester plate, the harvester plate having a plurality of wells recessed into one surface thereof, these wells conforming in size, number, and arrangement to the wells of the micro-culture plate which are of a conical shape with the wider openings of said conically shaped wells on one side of said micro-culture plate being of a diameter less than about one quarter of an inch which is a size sufficient for culturing biological samples while the micro-culture plate is in an inverted position and the narrower openings of said conically shaped wells being in open communication with fluid inlet passages which are open to the other side of said micro-culture plate, said wells of said culture plate having raised rims about the same and the wells of said harvester plate being such a size that they are a close fit for the rims when the wells of the two plates are brought together and the rims of the culture plate wells enter the mouths of the harvester plate wells, and the wells of the harvester plate capable of holding filter elements by virtue of their shape for absorbing liquid from corresponding wells of the culture plate when the wells of the culture plate, with the culture plate in an upside-down orientation, are contacted with corresponding wells of the harvester plate, and in which the harvester plate wells have fluid outlets at their bases. A method of culturing biological samples and harvesting the same, comprising: culturing biological liquid samples in a plurality of wells in a micro-culture plate while said micro-culture plate is in an inverted position, the wells in said micro-culture plate being of a conical shape with the wider openings of said conically shaped wells on one side of said microculture plate being of a diameter less than about one quarter of an inch and thus being sufficient for culturing said samples while said micro-culture plate is inverted, said wells having raised rims provided about the mouths of the same; mating said micro-culture plate after culturing with a harvester plate containing a plurality of wells recessed into one surface thereof, these wells conforming in size, number and arrangement to the wells of said micro-culture plate, being of such a size that they are a close fit for said rims when the wells of the micro-culture plate and the harvester plate are brought together and the rims of the culture plate wells enter the mouth of the harvester plate wells, and having fluid outlets at their bases and said harvester plate wells being capable of holding filter elements by virture of their shape for absorbing liquid from the corresponding wells in the culture plate; and filtering said samples in the wells of said micro-culture plate through said absorbent elements while the micro-culture plate is maintained in an inverted position.