초록 ▼

A method, test kit and labeled component for the detection and/or determination of one or more components of the reaction between a specific binding protein and the corresponding bindable substance, in which one or more labelled components are used, that are obtained by coupling particles of a dispe

A method, test kit and labeled component for the detection and/or determination of one or more components of the reaction between a specific binding protein and the corresponding bindable substance, in which one or more labelled components are used, that are obtained by coupling particles of a dispersion of a metal, metal compound or polymer nuclei, coated with a metal or metal compound, having a particle size of at least 5 nm, directly or indirectly to the desired component of said reaction. During the reaction or after an adequate reaction time, the physical properties and/or the amount of the metal and/or the formed metal containing agglomerate, is/are determined in the test sample, or optionally after a separation of the bound and free metal-labelled components in one of the derived fractions by methods known as known by those skilled in the immunoassay field.

대표청구항 ▼

The process for the detection and/or determination of one or more components of the reaction between a specific binding protein and the corresponding bindable substance in an aqueous test sample, by applying the known binding affinity of such components for one another, comprising: (a) employing one

The process for the detection and/or determination of one or more components of the reaction between a specific binding protein and the corresponding bindable substance in an aqueous test sample, by applying the known binding affinity of such components for one another, comprising: (a) employing one or more labelled components, obtained by coupling directly or indirectly the desired component of said reaction to particles of an aqueous sol dispersion of a metal, metal compound or polymer nuclei coated with a metal or metal compound, having a particle size of at least 5 nm, and (b) detecting and/or determining during the reaction or after an adequate reaction time and optionally after separation of the bound and free labelled components, the physical properties and/or the amount of the metal and/or a formed agglomerate containing said sol dispersed particles in the test sample or one of the derived fractions, which detection and/or determination provides a qualitative and/or quantitative indication of the component or components to be detected and/or determined. A test kit, to be used for the detection and/or determination of one or more components of the reaction between a specific binding protein and a corresponding bindable substance thereto in an aqueous medium according to a predetermined protocol, comprising: (a) a metal-labelled component which has been obtained by coupling a component of said reaction to particles of an aqueous sol dispersion of a metal, metal compound or polymer nuclei coated with a metal or metal compound, which parties have a size of at least 5 nm; (b) one or more additional immunochemical reagents of which at least one reagent is a ligand or an immobilized ligand, which ligand is selected from the group consisting of (aa) a ligand capable of binding with the metal-labelled component (a); (bb) a ligand capable of binding with a binding partner of the metal-labelled component (a); (cc) a ligand capable of binding with at least one of the component(s) to be determined; and (dd) a ligand capable of binding with at least one of the binding partners of at least one of the component(s) to be determined; and (c) directions for the performance of a protocol for the detection and/or determination of one or more components of an immunochemical reaction in an aqueous medium between a specific binding protein and a corresponding bindable substance thereto.