초록 ▼

The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affin

The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affinity chromatography matrix which remains an uncharged species at varying pH\s. The carbonylated product is also useful for the preparation of other compounds: Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose, typical macroporous synthetic polymers are acrylamides, acrylates and methacrylates, typical rigid supports are silica beads coated with hydroxy alkyl groups and typical cabonylating agents are N,N′-carbonyl diimidazole; N,N′carbonyl di-1,2,3-benzotriazole; and N,N′-carbonyl di-1,2,4-triazole.

대표청구항 ▼

A compound of the general formula III [Figure] III wherein MATRIX is a water and organic insoluble radical selected from the group consisting of: cross-linked polysaccharides, subsituted by C2-C8 alkyl or alkenyl groups, macroporous synthetic polymers, co-polymers of cross-linked substituted polysac

A compound of the general formula III [Figure] III wherein MATRIX is a water and organic insoluble radical selected from the group consisting of: cross-linked polysaccharides, subsituted by C2-C8 alkyl or alkenyl groups, macroporous synthetic polymers, co-polymers of cross-linked substituted polysaccharides with macroporous synthetic polymers, cross-linked co-polymers of unsubstituted polysaccharides with macroporous synthetic polymers, and rigid supports with pendant hydroxyalkyl groups, and Y is a member selected from the group consisting of: imidazolyl; 1,2,4-triazolyl; and 1,2,3-benzotriazolyl. The compound as claimed in claim 1 wherein said MATRIX is a copolymer of polyacrylamide with a cross-linked substituted or unsubstituted polysaccharide selected from the group consisting of: agarose, starch, dextran, cellulose, regenerated cellulose, hydroxy C2-C4 alkylated cellulose and hydroxy C2-C4 alkylated regenerated cellulose.