초록 ▼

In a photochemical immunological assay, an antigen or antibody is labelled with a spectral sensitizer or fogging agent for photographic use, and then an immune reaction is caused. The resulting reaction mixture is dropwise added to a multilayer analysis sheet. After either one of the labelled antige

In a photochemical immunological assay, an antigen or antibody is labelled with a spectral sensitizer or fogging agent for photographic use, and then an immune reaction is caused. The resulting reaction mixture is dropwise added to a multilayer analysis sheet. After either one of the labelled antigen or antibody (F) or the labelled antigen-antibody bound product (B) is brought into contact with silver halide by passing either one through a B/F separating layer provided in the multilayer analysis sheet, the analysis sheet is exposed with a light that the spectral sensitizer or fogging agent absorbs. Then, the resulting optical density obtained by development processing when a spectral sensitizer is used, or without development processing when a fogging agent is used is measured. The multilayer analysis sheet comprises a support having provided thereon a silver halide layer and a B/F separating layer and makes the photochemical immunological assay simpler and more accurate.

대표청구항 ▼

A method of performing a competitive immunoassay for a trace component in a sample comprising: (a) adding a labelled antigen or labelled antibody to said sample in a competitive immunoassay protocol, said label being selected from the group consisting of a spectral sensitizer and a fogging agent, (b

A method of performing a competitive immunoassay for a trace component in a sample comprising: (a) adding a labelled antigen or labelled antibody to said sample in a competitive immunoassay protocol, said label being selected from the group consisting of a spectral sensitizer and a fogging agent, (b) permitting the immune reactions to take place, (c) contacting the resulting reaction mixture with a multilayer analysis sheet comprising a support having provided thereon, in sequence, (1) a silver halide light sensitive layer and (2) a free labelled immunochemical/bound labelled immunochemical separating layer, (d) contacting either the free labelled immunochemical or the bound immunochemical which has passed through said separating layer with said silver halide light sensitive layer, said immunochemical being either antigen or antibody, (e) when said label is said spectral sensitizer, exposing the resultant product of step (d) to light having a wavelength which the spectral sensitizer label absorbs, (f) developing the multilayer analysis sheet; and (g) measuring the optical density resulting from step (f).