Deutsch Marshall E. (Sudbury MA) Mead Louis W. (Lexington MA)
출원인 / 주소
Thyroid Diagnostics, Inc. (Bedford MA 02)
인용정보
피인용 횟수 :
116인용 특허 :
5
초록▼
A test device for determining a characteristic of a sample, particularly for determining substances in fluid samples. The device comprises a strip element which is composed of a material capable of transporting a developing fluid therealong by capillarity and which has a portion at a predetermined l
A test device for determining a characteristic of a sample, particularly for determining substances in fluid samples. The device comprises a strip element which is composed of a material capable of transporting a developing fluid therealong by capillarity and which has a portion at a predetermined location on the strip element for receiving the test sample and portions at predetermined locations on the strip element incorporated with reagent means for providing a detectable response sensitive to the characteristic under determination. The beginning end portion of the strip element is immersed in the developing fluid which, as a result, traverses the length on the strip element, thereby promoting appropriate contact between the test sample and the reagent means resulting in the disposition of a detectable response at a predetermined location on the strip element, which response is a function of the characteristic under determination. The test device is particularly suited for performing binding assays, in particular those wherein a radioisotope is used as a label, such as radioimmunoassays.
대표청구항▼
A test strip for determining a characteristic of a sample, comprising a length of material capable of transporting a developing liquid therealong by capillarity, said strip including a first end, at which capillary transport begins; a second end, at which capillary transport ends; a plurality of zon
A test strip for determining a characteristic of a sample, comprising a length of material capable of transporting a developing liquid therealong by capillarity, said strip including a first end, at which capillary transport begins; a second end, at which capillary transport ends; a plurality of zones positioned between said first and second ends, said zones including a first zone for receiving said sample, a second zone impregnated with a first reagent capable of being transported along said strip by said developing liquid, a third zone downstream of said first and second zones impregnated with a second reagent, said first and second zones being spaced sufficiently from said first end to permit contact of said first end but not said first and second zones with said developing liquid, said first reagent being capable of reacting with one of the following group consisting of (1) said sample, (2) said sample and said second reagent, and (3) said second reagent in competition with said sample, to form a product in an amount dependent on said characteristic being determined, one of the group consisting of said second reagent and said material being a retarding element capable of slowing capillary transport of a moving element, said moving element being one of the group consisting of said product and said first reagent to thereby separate said product from said first reagent, and said first reagent having a detectable property that provides a label for said first reagent and for said product to permit one of them to be a detectable agent whose amount is to be detected; and a measuring location at or downstream of said third zone, said measuring location being spaced upstream of said second end a distance preselected so that, when transport of said developing liquid along said strip element is terminated by the leading front thereof reaching said second end, said product and said first reagent have been separated sufficiently to permit the amount of said detectable agent to be detected at said location by measuring said detectable property, whereby, after said sample is placed on said first zone and said first end is dipped into said developing liquid, said developing liquid transports said sample and first reagent to bring about the reaction to form said product, in an amount dependent on said characteristic; said retarding element slows transport of said moving element to spatially separate the two; and the amount of said moving element and thereby said characteristic, is measured at said measurement location by measuring said detectable property.
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