초록 ▼

A process for recovering diterpene glycosides from the Stevia rebaudiana plant includes the steps of sequentially extracting plant material with a first solvent of intermediate polarity to extract plant substances which tend to interfere with a liquid chromatographic separation of the glycosides, an

A process for recovering diterpene glycosides from the Stevia rebaudiana plant includes the steps of sequentially extracting plant material with a first solvent of intermediate polarity to extract plant substances which tend to interfere with a liquid chromatographic separation of the glycosides, and then with a second solvent of high polarity to extract glycosides, and chromatographically separating the extracted glycosides by introducing them onto a liquid chromatography column having a packing of an oxygen-containing organic stationary phase covalently bonded through a silicon atom to an inorganic support, eluting them with a solvent of polarity higher than that of the first solvent but lower than that of the second solvent, and collecting individually eluate fractions rich in respective glycosides.

대표청구항 ▼

A process for recovering diterpene glycosides from Stevia rebaudiana plant material, said process comprising: sequentially extracting Stevia rebaudiana plant material with a first solvent of intermediate polarity to remove impurities therefrom in a first extract substantially free of diterpene glyco

A process for recovering diterpene glycosides from Stevia rebaudiana plant material, said process comprising: sequentially extracting Stevia rebaudiana plant material with a first solvent of intermediate polarity to remove impurities therefrom in a first extract substantially free of diterpene glycosides, and then with a second solvent of high polarity to give a second extract containing diterpene glycosides; chromatographing the resulting extracted diterpene glycosides on a liquid chromatography column having a packing comprising an oxygen-containing organic stationary phase covalently bonded through a silicon atom to an inorganic support; said extracted glycosides being introduced onto said column for chromatography thereon in solution in said second solvent or in another solvent having a high polarity not substantially greater than the polarity of the second solvent; said column being eluted with a solvent of polarity higher than that of said first solvent and lower than that of said second solvent; and collecting individually eluate fractions rich in respective diterpene glycosides.