초록 ▼

In particle agglutination immunoassays for an analyte (e.g. an antigen or antibody) in a liquid sample (e.g. human serum), interferences arise due to non-specific protein-protein interactions and the like. These interferences are reduced or overcome by including in the assay mixture a chaotropic or

In particle agglutination immunoassays for an analyte (e.g. an antigen or antibody) in a liquid sample (e.g. human serum), interferences arise due to non-specific protein-protein interactions and the like. These interferences are reduced or overcome by including in the assay mixture a chaotropic or chaotropic-like agent, in a carefully controlled amount. Such agents include guanidine, guanidinium hydrochloride or thiocyanate, sodium or ammonium thiocyanate, and urea, sodium chloride and ethylenediamine tetraacetic acid.

대표청구항 ▼

A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination or the particles occurs to an extent dependent on the amount of analyte present, and dete

A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination or the particles occurs to an extent dependent on the amount of analyte present, and determining said extent and thereby the amount of analyte present, wherein the mixture also includes one or more agents to reduce non-specific protein interaction interferences, the amount of said agent(s) being sufficient to reduce the effect on agglutination of said interferences, wherein said agents comprise at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide. A particle agglutination specific binding assay composition for the non-specific protein interaction interference-free determination of an analyte in a liquid sample, which composition comprises: a particle to which is bound protein and a specific binding partner for said analyte; and an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.