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특허 상세정보

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Immunometric assays using monoclonal antibodies

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) G01N-033/54    G01N-033/56   
미국특허분류(USC) 436/513 ; 435/7 ; 436/548 ; 436/529
출원번호 US-0175133 (1980-08-04)
발명자 / 주소
출원인 / 주소
인용정보 피인용 횟수 : 818  인용 특허 : 2
초록

“Two-site”or “sandwich”immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies. One monoclonal antibody is presented in a soluble labeled form and a second monoclonal antibody is presented bound to a solid carrier; the soluble and bound monoclonal antibodies may be the products of either the same or different cell lines. Each monoclonal antibody has an affinity for the antigenic substances of at least about 108 liters/mole.

대표
청구항
▼

A process for the determination of the presence of concentration of an antigenic substance in a fluid comprising the steps: (a) contacting a sample of the fluid with a measured amount of a soluble first monoclonal antibody to the antigenic substance in order to form a soluble complex of the antibody and antigenic substance present in said sample, said first monoclonal antibody being labelled; (b) contacting the soluble complex with a second monoclonal antibody to the antigenic substance, said second monoclonal antibody being bound to a solid carrier, sai...

이 특허에 인용된 특허 (2)

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  2. Piasio ; Roger N. ; Ryan ; James W. ; Woiszwillo ; James E.. Reverse sandwich immunoassay. USP1978074098876.

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