초록 ▼

An enclosed device and method for controlled freezing of cell cultures, especially eucaryotic cell cultures, contained in cell culture vials by means of a conventional freezer includes a container and top, a supporting grid and supporting grid base within the container for supporting the cultire via

An enclosed device and method for controlled freezing of cell cultures, especially eucaryotic cell cultures, contained in cell culture vials by means of a conventional freezer includes a container and top, a supporting grid and supporting grid base within the container for supporting the cultire vials and a compatible liquid refrigerant in a volume of at least fifteen times the culture volume and at a level to totally immerse all of the cultures to be frozen. Compatible liquid refrigerants include the straight chain aliphatic alcohols, preferrably ethanol and methanol.

대표청구항 ▼

A device for controlled freezing of cell cultures contained in a portion of one or more cell culture vials which comprises: (a) a container having a removable lid and a bottom; (b) grid means for independently supporting each cell culture vial in a substantially upright position in said container; (

A device for controlled freezing of cell cultures contained in a portion of one or more cell culture vials which comprises: (a) a container having a removable lid and a bottom; (b) grid means for independently supporting each cell culture vial in a substantially upright position in said container; (c) a volume of liquid refrigerant in said container sufficient to completely submerse said portion of each vial containing cell culture in said refrigerant, said volume of refrigerant being in excess of fifteen times the total volume of cell culture contained in said one or more vials; and (d) suspension means resting on the bottom of said container to suspend said grid means and said one or more vials above the bottom of said container.