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특허 상세정보

Device and method for controlled freezing of cell cultures

국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) F25D-003/08   
미국특허분류(USC) 62/457 ; 62/78 ; 435/284
출원번호 US-0283634 (1981-07-15)
발명자 / 주소
출원인 / 주소
인용정보 피인용 횟수 : 16  인용 특허 : 4

An enclosed device and method for controlled freezing of cell cultures, especially eucaryotic cell cultures, contained in cell culture vials by means of a conventional freezer includes a container and top, a supporting grid and supporting grid base within the container for supporting the cultire vials and a compatible liquid refrigerant in a volume of at least fifteen times the culture volume and at a level to totally immerse all of the cultures to be frozen. Compatible liquid refrigerants include the straight chain aliphatic alcohols, preferrably ethano...


A device for controlled freezing of cell cultures contained in a portion of one or more cell culture vials which comprises: (a) a container having a removable lid and a bottom; (b) grid means for independently supporting each cell culture vial in a substantially upright position in said container; (c) a volume of liquid refrigerant in said container sufficient to completely submerse said portion of each vial containing cell culture in said refrigerant, said volume of refrigerant being in excess of fifteen times the total volume of cell culture contained ...

이 특허를 인용한 특허 피인용횟수: 16

  1. Glascock Battle (Knoxville TN). Apparatus and process for quick freezing of blood plasma. USP1988124790141.
  2. Pring John B. (17 Riverside ; Folly Island Hertfort ; Herts SG13 GB2). Container for transport of frozen materials such as biological samples. USP1990124974423.
  3. Patrick L. Mullens ; Gregg Emmel ; Kevin Giesy ; Christy Thomas. Cryogenic shipping container. USP2002106467642.
  4. Jimenez-Rios, Jorge L.. Cryogenic storage container with sealing closure and methods of using the same. USP2016129518898.
  5. Jimenez-Rios, Jorge L.; Woodard, Bryan D.; Nichols, Shawn; Philhower, Scott K.; Hoult, Barry B.. Cryogenic storage container, storage device, and methods of using the same. USP2016039297499.
  6. Grischenko Valentin I. (ulitsa Pushkinskaya ; 67/69 ; kv. 31 Kharkov SUX) Tarasov Valery F. (ulitsa S. Esenina ; 19 ; kv. 40 Kharkov SUX) Galchenko Sergei E. (ulitsa Korchagintsev ; 36 ; kv. 17 Khark. Device for refrigeration and freezing of biological objects. USP1989044817397.
  7. Delatte Daniel (Saint-Maur FRX). Device for storing tubes in a cryogenic container. USP1987054665713.
  8. Chopas Nicholas J. (Germantown MD). Enzyme cooler with porous foam refrigerant block. USP1997115689970.
  9. Frederick S. Bucholz. Insulated container. USP2002066405556.
  10. Julie Ann Wlodarski. Medical specimen tote. USP2002036361746.
  11. Sakao Nobuo (Sapporo JPX) Kuraoka Yasuo (Sapporo JPX). Method of freezing fertilized ova, spermatozoa or the like and apparatus therefor. USP1984124487033.
  12. Liu Lee-Cheng ; Lai Shoupeng. Method of producing adenoviral vector stocks. USP2001016168941.
  13. Fuller, Jess Paul; Pegg, David; Bird, Robert McLean; Clifford, Timothy Burgess; Clayson, Tony. Preparation of porous silicone rubber for growing cells or living tissue. USP2005056900055.
  14. Henning Steve (2734 Carmel Woods Dr. Seabrook TX 77586) Camp James A. (1713 Asbury La. Deer Park TX 77536). Shipper. USP1997025600958.
  15. Cutting, Jonathan; Gay, Isabelle; Reif, Oscar Werner. Systems and methods for use in freezing, thawing, and storing biopharmaceutical materials. USP2013058448457.
  16. Giesy, R. Kevin; Ventura, Mark; Roberts, Funn. Vapor plug for cryogenic storage vessels. USP2003046539726.