초록 ▼

An antigen or antibody and an immunochemically neutral protein are immobilized in two stages on a microporous membrane coated with a protein such as zein or collagen. Immobilized antigen to Toxoplasma gondii is used in an immunoassay to detect the presence of antibody to Toxoplasma gondii in serum.

An antigen or antibody and an immunochemically neutral protein are immobilized in two stages on a microporous membrane coated with a protein such as zein or collagen. Immobilized antigen to Toxoplasma gondii is used in an immunoassay to detect the presence of antibody to Toxoplasma gondii in serum. Antibody that becomes bound to the immobilized antigen is detected with an antibody- enzyme conjugate. Immobilizing the immunochemically netural protein on the membrane in a second stage immobilization step minimizes nonspecific binding during the immunoassay.

대표청구항 ▼

A method of nonimmunochemically immobilizing two proteins on the internal and external surfaces of a microporous membrane having pore sizes in the range from 25 nanometers to 25 micrometers in its uncoated state, comprising: coating the internal and external surfaces of the membrane with a protein s

A method of nonimmunochemically immobilizing two proteins on the internal and external surfaces of a microporous membrane having pore sizes in the range from 25 nanometers to 25 micrometers in its uncoated state, comprising: coating the internal and external surfaces of the membrane with a protein selected from the group consisting of zein and collagen, to provide a coated membrane that is at least 50% microporous, immersing the coated membrane in a solution of a biologically active first protein selected from the group consisting of the antigen of and the antibody to Toxoplasma gondii, in order to immobilize the first protein, then immersing the coated membrane having the biologically active first protein immobilized thereon in a solution of an immunochemically neutral second protein selected from the group consisting of fetal calf serum and bovine gamma globulin fraction II, in order to immobilize the second protein while the first protein remains immobilized and retains its biological activity.