초록 ▼

Method and apparatus for molecular spectroscopy, particularly for the determination of products of metabolism. The absorption of infrared radiation by a specimen (2) which contains the substance to be determined is measured. The absorption is measured simultaneously at two different wavelengths (l1,

Method and apparatus for molecular spectroscopy, particularly for the determination of products of metabolism. The absorption of infrared radiation by a specimen (2) which contains the substance to be determined is measured. The absorption is measured simultaneously at two different wavelengths (l1, l2), the first wavelength (l1) being so selected that upon changes in concentration of the substance to be determined in the specimen (2) only a negligibly small change, if any, of the radiation absorption takes place, while the second wavelength (l2) lies in the region of a substance-specific absorption band of the substance to be determined. After measurement of the radiation intensities (I1 and I2) at the two wavelengths (l1, l2) the signal is standardized by formation of the quotient (I2/I1). The method makes it possible to effect a quantitative reproducible measurement in the natural biological medium or in dialysates without pretreatment of the specimen and with only small amounts of substance. By suitable selection of the second wavelength (l2) a plurality of substances can be determined.

대표청구항 ▼

A method for the spectroscopic determination of glucose concentration in an unpretreated multi-component specimen selected from the group of whole blood and urine which comprises the steps of: (a) simultaneously measuring the absorption values of infrared radiation by said specimen at a first wavele

A method for the spectroscopic determination of glucose concentration in an unpretreated multi-component specimen selected from the group of whole blood and urine which comprises the steps of: (a) simultaneously measuring the absorption values of infrared radiation by said specimen at a first wavelength lying within the infrared spectral range of 940 to 950 cm-1 and a second wavelength lying within the infrared spectral range of 1090 to 1095 cm-1; and (b) standardizing the measurement by forming the quotient of the absorption values of the first and second wavelengths: whereby the glucose concentration is proportional to the absorption value measured at second wavelength, and the absorption value measured at said first wavelength is essentially independent of said concentration.