초록 ▼

A multiple component binding assay system, and related methods of making and using it, having a plurality of coated filaments mounted at their opposite ends on a support, in a predetermined spaced relationship, for simultaneously screening a liquid test sample for a plurality of components. The syst

A multiple component binding assay system, and related methods of making and using it, having a plurality of coated filaments mounted at their opposite ends on a support, in a predetermined spaced relationship, for simultaneously screening a liquid test sample for a plurality of components. The system is particularly suitable for screening the sample for IgE class antibodies specific for certain allergens. The filaments are preferably cotton threads, each thread binding a different allergen through a covalent bond. The support, which is preferably cut away adjacent the mid-portion of each filament to reduce background interference, is composed of a plastic or other water insoluble material, permitting inexpensive and relatively simple manufacture. After any IgE class antibodies present in the liquid sample have reacted with the corresponding allergens coated on the filaments, they are detected using second antibodies labeled with radioactive, fluorescent or enzymatic tracers. By using a high energy radioactive tracer, such as 125I, autoradiographic techniques with phosphor screen enhancement allow for diagnostic analysis by a densitometer or even the unaided human eye.

대표청구항 ▼

For use in the diagnostic analysis of a liquid specimen through binding assays, a diagnostic system comprising a plurality of filaments supported in a spaced relationship for simultaneous contact with said liquid specimen, at least two of said filaments being coated with binding assay components fro

For use in the diagnostic analysis of a liquid specimen through binding assays, a diagnostic system comprising a plurality of filaments supported in a spaced relationship for simultaneous contact with said liquid specimen, at least two of said filaments being coated with binding assay components from different assays, for multiple, coincident measurement of said assays. A method of detecting the presence of multiple biological agents in a liquid specimen through binding assays, comprising steps of: incubating the liquid specimen with a diagnostic device having a plurality of filaments secured to a support member, at least two of the filaments being coated with binding assay components that react with different biological agents that might be present in the specimen; separating the liquid specimen and the diagnostic device from each other; incubating the diagnostic device with a solution containing labeled components that will bind to any of the biological agents that might have bound to the binding assay components in the first step of incubating; separating the diagnostic device and the solution from each other; and positioning the diagnostic device proximate to a photographic film, to expose a pattern in the film indicative of the presence of labeled components on each of the filaments.