초록 ▼

Two-site immunometric assays for multideterminant antigens are described in which the antigen is reacted with an immobilized monoclonal antibody directed against one antigen determinant and a second monoclonal antibody that is directed against a distinct antigenic determinant and is of a different c

Two-site immunometric assays for multideterminant antigens are described in which the antigen is reacted with an immobilized monoclonal antibody directed against one antigen determinant and a second monoclonal antibody that is directed against a distinct antigenic determinant and is of a different class or subclass than the immobilized monoclonal antibody. The second monoclonal antibody is labeled in direct versions of the assay and is reacted with a labeled antibody against it in indirect versions of the assay. The immobilizing medium and classes (subclasses) of the antibodies may be selected so as to reduce the likelihood of nonspecific binding enhance sensitivity and/or permit signal amplification.

대표청구항 ▼

In a two-site immunoassay for detecting the presence of a multideterminant antigen in a sample wherein the antigen in the sample is reacted with (a) an immobilized monoclonal antibody directed aganist one of the antigen determinants; and (b) a second monoclonal antibody that is (i) directed against

In a two-site immunoassay for detecting the presence of a multideterminant antigen in a sample wherein the antigen in the sample is reacted with (a) an immobilized monoclonal antibody directed aganist one of the antigen determinants; and (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; and (ii) labeled directly or indirectly; the improvement wherein the second monoclonal antibody is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody. The immunoassay of claim 1 wherein the second monoclonal antibody is of a class or subclass of antibody that may be removed selectively from the immobilizing medium to which the immobilized monoclonal antibody is attached without substantial removal of the immobilized antibody from the immobilizing medium.