초록 ▼

A layer of bioactive molecules is coated on a dielectric substrate and is contacted with a solution to be analyzed containing a complex conjugate of said molecule. The rate of complexion of said conjugate moiety with the layer which is a function of its concentration in the analyte is measured by op

A layer of bioactive molecules is coated on a dielectric substrate and is contacted with a solution to be analyzed containing a complex conjugate of said molecule. The rate of complexion of said conjugate moiety with the layer which is a function of its concentration in the analyte is measured by optical means and correlated with corresponding rates obtained from standard reference measurements, thus providing data for determining the unknown concentration of said conjugate moiety in the solution to be analyzed.

대표청구항 ▼

A bioassay method for determining a bioactive substance in a sample by the reaction of said bioactive substance, antigen AG or antibody AB, with its specific binding partner, antibody AB or antigen AG, respectively, which comprises contacting with the sample to be determined a transparent dielectric

A bioassay method for determining a bioactive substance in a sample by the reaction of said bioactive substance, antigen AG or antibody AB, with its specific binding partner, antibody AB or antigen AG, respectively, which comprises contacting with the sample to be determined a transparent dielectric substrate the surface of which is at least partly coated with a film of or containing AB or AG, respectively distributed on the surface of said transparent dielectric substrate, ellipsometrically measuring the rate of optical change occurring in a beam of light where reflection takes place at the boundary line between substrate surface and film as the result of the reaction of AB and the AG of the sample, then correlating the rate data thus obtained with standard rate data from identical rate measurements done with known standard samples of AG or AB, respectively.