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In a method for assaying an analyte comprising applying a liquid sample containing an analyte to a spreading layer of a multilayer analysis element comprising a support having laminated thereon at least one reagent layer containing a substrate capable of converting a detectable chemical species upon

In a method for assaying an analyte comprising applying a liquid sample containing an analyte to a spreading layer of a multilayer analysis element comprising a support having laminated thereon at least one reagent layer containing a substrate capable of converting a detectable chemical species upon reaction with the analyte and a liquid sample-spreading layer and then detecting the chemical species, a wetting liquid is applied onto the spreading layer, prior to applying the liquid sample to the spreading layer. A liquid sample having a high analyte content can be measured with high accuracy.

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1. In a method for assaying an analyte comprising applying a liquid sample having a relatively high viscosity containing the analyte onto a spreading layer of a multilayer analysis element comprising a support having laminated thereon, in sequence, at least one reagent layer containing a substance c

1. In a method for assaying an analyte comprising applying a liquid sample having a relatively high viscosity containing the analyte onto a spreading layer of a multilayer analysis element comprising a support having laminated thereon, in sequence, at least one reagent layer containing a substance capable of converting a detectable chemical species upon reaction with said analyte and a liquid sample-spreading layer and then detecting said detectable chemical species, the improvement which comprises applying a wetting liquid onto said spreading layer of the multilayer analysis element prior to applying said liquid sample to the multilayer analysis element, said liquid sample being applied to the multilayer analysis element while at least the surface of said spreading layer is maintained wet with said wetting liquid. 2. The method of claim 1 wherein said wetting liquid comprises (1) water, (2) glycerin, (3) an alcohol, (4) a polar solvent other than water or (5) a mixture of glycerin, an alcohol or a polar solvent with water. 3. The method of claim 2 wherein said alcohol is methanol, ethanol or propanol. 4. The method of claim 2 wherein said polar solvent is acetone or tetrahydrofuran. 5. The method of claim 2 wherein said mixture is a mixture of glycerin and water. 6. The method of claim 2 wherein the mixing ratio of (2), (3) or (4) with water is at least 0.1 vol%, based on 100 vol% of water. 7. The method of claim 2 wherein said wetting liquid is contained in an amount of about 0.1 to about 20 μl per 1 cm 2 of said spreading layer. 8. The method of claim 7 wherein said wetting liquid contains a water-soluble additive. 9. The method of claim 8 wherein said additive is selected from the group consisting of an organic or inorganic salt, a surface active agent, a buffer, an organic or inorganic alkali, an acid, a high molecular weight substance and an oxidation-reduction substance. 10. The method of claim 9 wherein said organic or inorganic salt is selected from the group consisting of sodium chloride, a phosphate and calcium chloride. 11. The method of claim 9 wherein said surface active agent is an anionic suface active agent, a cationic surface active agent or a nonionic surface active agent. 12. The method of claim 9 wherein said buffer is selected from the group consisting of a Na 2 HPO 4 --KH 2 PO 4 buffer solution, a Na 2 HPO 4 --citric acid buffer solution and a tris(hydroxymethyl)aminomethane-hydrochloride buffer solution. 13. The method of claim 9 wherein said high molecular weight substance is protein. 14. The method of claim 13 wherein said protein is albumin. 15. The method of claim 9 wherein said organic or inorganic acid is selected from the group consisting of citric acid, acetic acid, phosphoric acid, hydrochloric acid and tartaric acid. 16. The method of claim 9 wherein said organic or inorganic alkali is selected from the group consisting of sodium hydroxide, sodium carbonate, sodium bicarbonate, ammonia and triethylamine. 17. The method of claim 9 wherein said oxidation-reduction substance is ascorbic acid. 18. The method of claim 1, wherein said wetting liquid is contained in an amount of at least 0.1 μl per 1 cm 2 of the spreading layer. 19. The method of claim 18, wherein said wetting liquid is contained in an amount of 0.1 to 20 μl per 1 cm 2 of the spreading layer. 20. The method of claim 19, wherein said wetting liquid is contained in an amount of 0.5 to 20 μl per 1 cm 2 of the spreading layer. 21. The method of claim 1, wherein said liquid sample has a viscosity of about 20 to 50 cps, measured at 25° C. 22. The method of claim 1, wherein said liquid sample is applied to the spreading layer within 30 seconds after application of wetting liquid. 23. The method of claim 5 wherein the mixing ratio of glycerin with water is at least 0.1 vol%, based on 100 vol% of water.