A process is provided for the preparation of magnetic particles to which a wide variety of molecules may be coupled. The magnetic particles can be dispersed in aqueous media without rapid settling and conveniently reclaimed from media with a magnetic field. Preferred particles do not become magnetic
A process is provided for the preparation of magnetic particles to which a wide variety of molecules may be coupled. The magnetic particles can be dispersed in aqueous media without rapid settling and conveniently reclaimed from media with a magnetic field. Preferred particles do not become magnetic after application of a magnetic field and can be redispersed and reused. The magnetic particles are useful in biological systems involving separations.
대표청구항▼
1. A coupled magnetically-responsive particle comprising a magnetic metal oxide core generally surrounded by a coat of polymeric silane to which molecules of at least one type of bioaffinity adsorbent are covalently coupled, a mass of the uncoupled particles being dispersable in aqueous media to for
1. A coupled magnetically-responsive particle comprising a magnetic metal oxide core generally surrounded by a coat of polymeric silane to which molecules of at least one type of bioaffinity adsorbent are covalently coupled, a mass of the uncoupled particles being dispersable in aqueous media to form an aqueous dispersion having (a) a fifty-percent-turbidity-decrease settling time of greater than about 1.5 hours in the absence of a magnetic field, and (b) a ninety-five-percent-turbidity-decrease separation time of less than about 10 minutes in the presence of a magnetic field, the magnetic field being applied to the aqueous dispersion by bringing a vessel containing a volume of the dispersion into contact with a pole face of a permanent magnet, the permanent magnet having a volume which is less than the volume of the aqueous dispersion in the vessel. 2. A coupled magnetically-responsive particle comprising a superparamagnetic iron oxide core generally surrounded by a coat of polymeric silane to which molecules of at least one type of bioaffinity adsorbent are covalently coupled, the iron oxide core including a group of crystals of iron oxide, the uncoupled particle having a mean diameter as measured by light scattering between about 0.1μ and about 1.5μ and a surface area as measured by nitrogen gas adsorption of at least about 100 m 2 /gm, a mass of the uncoupled particles being dispersable in aqueous media to form an aqueous dispersion having (a) a fifty-percent-turbidity-decrease settling time of greater than about 1.5 hours in the absence of a magnetic field, and (b) a ninety-five-percent-turbidity-decrease separation time of less than about 10 minutes in the presence of a magnetic field, the magnetic field being applied to the aqueous dispersion by bringing a vessel containing a volume of the dispersion into contact with a pole face of a permanent magnet, the permanent magnet having a volume which is less than the volume of the aqueous dispersion in the vessel. 3. A coupled magnetically-responsive particle comprising a ferromagnetic metal oxide core generally surrounded by a coat of polymeric silane to which molecules of at least one type of bioaffinity adsorbent are covalently coupled, the metal oxide core including a group of crystals of metal oxide, the uncoupled particle having a mean diameter as measured by light scattering between about 0.1μ and about 1.5μ and a surface area as measured by nitrogen gas adsorption of at least about 100 m 2 /gm, a mass of the uncoupled particles being dispersable in aqueous media to form an aqueous dispersion having (a) a fifty-percent-turbidity-decrease settling time of greater than about 1.5 hours in the absence of a magnetic field, and (b) a ninety-five-percent-tubidity-decrease separation time of less than about 10 minutes in the presence of a magnetic field, the magnetic field being applied to the aqueous dispersion by bringing a vessel containing a volume of the dispersion into contact with a pole face of a permanent magnet, the permanent magnet having a volume which is less than the volume of the aqueous dispersion in the vessel. 4. The coupled magnetically-responsive particle of claim 1, 2 or 3 wherein the bioaffinity adsorbent is selected from the group consisting of antibodies, antigens, haptens, enzymes, apoenzymes, enzymatic substrates, enzymatic inhibitors, cofactors, binding proteins, carrier proteins, compounds bound by binding proteins, compounds bound by carrier proteins, lectins, monosaccharides, polysaccharides, hormones, receptors, repressors and inducers. 5. The coupled magnetically-responsive particle of claim 2 or 3 wherein the covalently coupled bioaffinity adsorbent is an antibody selected from the group consisting of anti-thyroxine, anti-triiodothyronine, anti-thyroid stimulating hormone, anti-thyroid binding globulin, anti-thyroglobulin, anti-digoxin, anti-cortisol, anti-insulin, anti-theophylline, anti-vitamin B 12, anti-folate, anti-ferritin, anti-human chorionic gonadotropin, anti-follicle stimulating hormone, anti-leuteinizing hormone, anti-progesterone, anti-testosterone, anti-estriol, anti-estradiol, anti-prolactin, anti-human placental lactogen, anti-gastrin and anti-human growth hormone antibodies. 6. The coupled magnetically-responsive particle of claim 2 wherein the coat of polymeric silane is a coat of p-aminophenyltrimethoxysilane polymer and wherein the bioaffinity adsorbents are anti-thyroxine antibodies, which antibodies are covalently coupled to the coat of polymeric silane by diazotization. 7. The coupled magnetically-responsive particle of claim 2 wherein the coat of polymeric silane is a coat of p-aminophenyltrimethoxysilane polymer and wherein the bioaffinity adsorbents are anti-theophylline antibodies, which antibodies are covalently coupled to the coat of polymeric silane by diazotization. 8. The coupled magnetically-responsive particle of claim 2 wherein the coat of polymeric silane is a coat of carboxylic-acid terminated glutaric-anhydride-treated 3-aminopropyltrimethoxysilane polymer and wherein the bioaffinity adsorbents are molecules of vitamin B 12 binding protein, which molecules of vitamin B 12 binding protein are covalently coupled to the coat of polymeric silane by carbodiimide coupling. 9. The coupled magnetically-responsive particle of claim 2 wherein the coat of polymeric silane is a coat of N-2-aminoethyl-3-aminopropylsilane polymer and wherein the bioaffinity adsorbents are anti-triiodothyronine antibodies, which antibodies are covalently coupled to the coat of polymeric silane by glutaraldehyde coupling. 10. The coupled magnetically-responsive particle of claim 2 wherein the coat of polymeric silane is a coat of N-2-aminoethyl-3-aminopropylsilane polymer and wherein the bioaffinity adsorbents are anti-thyroid stimulating hormone antibodies, which antibodies are covalently coupled to the coat of polymeric silane by glutaraldehyde coupling. 11. The coupled magnetically-responsive particle of claim 2 wherein the coat of polymeric silane is a coat of N-2-aminoethyl-3-aminopropylsilane polymer and wherein the bioaffinity adsorbents are enzymes selected from the group consisting of alkaline phosphatase and β-galactosidase, which enzymes are covalently coupled to the coat of polymeric silane by glutaraldehyde coupling.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (32)
Forrest Gordon C. (Chelmsford GB2) Jay Ronald F. (New Malden GB2) Clements John A. (Wallington GB2), Automatic apparatus and method for the assay of fluid samples.
Widder Kenneth J. (8 E. Pearson St. Chicago IL 60611) Senyei Andrew E. (8 E. Pearson St. Chicago IL 60611), Intravascularly-administrable, magnetically-localizable biodegradable carrier.
Smith Kendall O. (133 Trillium La. San Antonio TX 78213) Gehle Warren D. (6815 Forest Haven San Antonio TX 78240), Magnetic attraction transfer process for use in solid phase radioimmunoassays and in other assay methods.
Senyei Andrew E. (Chicago IL) Widder Kenneth J. (Chicago IL), Method of incorporating water-soluble heat-sensitive therapeutic agents in albumin microspheres.
Rao, Galla Chandra; Larson, Christopher; Repollet, Madeline; Rutner, Herman; Terstappen, Leon W. M. M.; O'Hara, Shawn Mark; Gross, Steven, Analysis of circulating tumor cells, fragments, and debris.
Rao, Galla Chandra; Larson, Christopher; Repollet, Madeline; Rutner, Herman; Terstappen, Leon W. M. M.; O'Hara, Shawn Mark; Gross, Steven, Analysis of circulating tumor cells, fragments, and debris.
Leland Jonathan K. ; Shah Haresh P. ; Kenten John Henry ; Goodman Jack E. ; Lowke George E. ; Namba Yuzaburo,JPX ; Blackburn Gary F. ; Massey Richard J., Apparatus for improved luminescence assays.
Massey Richard J. ; Blackburn Gary F. ; Wilkins Elizabeth W. ; Leland Jonathan K., Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence.
Alfadul, Sulaiman M.; Alabdula'aly, Abdulrahman I.; Khan, Mujahid A., Apparatus for removing impurities from aqueous fluids using magnetic extractants.
Alfadul, Sulaiman M.; Alabdula'aly, Abdulrahman I.; Khan, Mujahid A., Apparatus for removing impurities from aqueous fluids using magnetic extractants.
Carey Glen A. (Grafton OH) Lewis Scott C. (Amherst OH) Mann Raymond A. (Kipton OH) Whitesel Mary B. (Grafton OH) Polaniec James P. (North Ridgeville OH) Woyansky George J. (Westlake OH) Pabst Stefan , Automated analyzer with reagent agitating device.
Groman Ernest V. (Brookline MA) Josephson Lee (Arlington MA) Lewis Jerome M. (Newton MA), Biodegradable superparamagnetic metal oxides as contrast agents for MR imaging.
Liberti Paul A. ; Rao Galla C. ; Chiarappa Joseph N., Coated, resuspendable magnetically responsive, transition metal oxide particles and method for the preparation thereof.
Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Compositions for isolating a target analyte from a heterogeneous sample.
Michael Bancroft Simmonds ; Kurt Gordon Jensen ; Jost Hermann Diederichs ; Randall Christopher Black, Computer program for making measurements of accumulations of magnetic particles.
Levinthal Cyrus (New York NY) Fine Richard M. (New York NY), Computing device for calculating energy and pairwise central forces of particle interactions.
Carey Glen A. (Grafton OH) Lewis Scott C. (Amherst OH) Mann Raymond A. (Kipton OH) Whitesel Mary Beth (Grafton OH) Polaniec James P. (North Ridgeville OH) Woyansky George J. (Westlake OH) Pabst Stefa, Constant luminescence source module for an automated analyzer.
Leland, John K.; Shah, Haresh P.; Kenten, John H.; Goodman, Jack E.; Lowke, George E.; Namba, Yuzaburo; Blackburn, Gary F.; Massey, Richard J., Electrochemiluminescent localizable complexes for assay compositions.
Duguet, Etienne; Mornet, Stéphane; Portier, Joseph, Ferrofluids stable in neutral media and modified ferrofluids modifies obtained by modification of the surface of the particles of said ferrofluids.
Uzan Michel,FRX ; Gicquel Thierry,FRX ; Lentwojt Edouard,FRX ; Marminio Dario,FRX, Immunodiagnostic reagent for carrying out a multi-stage immunoassay of at least one biological substance in a plurality.
Bushart, Sean P.; Bradbury, David; Elder, George Richard, Magnetic molecules: a process utilizing functionalized magnetic ferritins for the selective removal of contaminants from solution by magnetic filtration.
Miltenyi Stefan (Bergisch Gladbach DEX) Dority Douglas Bryan (Mill Valley CA) Phi-Wilson Janette (Los Altos Hills CA), Magnetic separation apparatus and method.
Owen Charles S. (Swarthmore PA) Silvia John C. (Warminster PA) D\Angelo Louis (Berlin NJ) Liberti Paul A. (Churchville PA), Magnetic-polymer particles.
Michael Bancroft Simmonds ; Kurt Gordon Jensen ; Jost Hermann Diederichs ; Randall Christopher Black, Method and apparatus for making measurements of accumulations of magnetically susceptible particles combined with analytes.
Simmonds, Michael Bancroft; Jensen, Kurt Gordon; Diederichs, Jost Hermann; Black, Randall Christopher, Method and apparatus for making measurements of patterns of magnetic particles in lateral flow membranes and microfluidic systems.
Arnquist David C. ; Barnes ; III Grady ; Button Richard D. ; Dunn Chadwick M. ; East ; Jr. Richard C. ; Fritchie Patrick P. ; Galitz Charles M. ; Gardner Gregory E. ; Grandone Cass J. ; Gray Robert C, Method for determination of item of interest in a sample.
Kleiber,J��rg; Walter,Thomas; Harttig,Herbert; Lesniak,Christop; Mennig,Martin; Riedling,Michael; Schmidt,Helmut, Method for separating biological material from a fluid using magnetic particles.
Schiestel, Thomas; Mueller, Thomas; Schirra, Hermann; Schmidt, Helmut, Method for separating components from liquid and gaseous media with nanocomposites.
Arnquist David C. ; Barnes ; III Grady ; Dunn Chadwick M. ; East ; Jr. Richard C. ; Fritchie Patrick P. ; Gardner Gregory E. ; Grandone Cass J. ; Gray Robert C. ; Holen James T. ; McCoy Jimmy D. ; Mi, Method of performing a process for determining an item of interest in a sample.
Barnardo, Martin C. N. M.; Harmer, Andrea W.; Bunce, Michael; Vaughan, Robert W.; Welsh, Kenneth I., Method of producing a body fluid sample depleted of anti-MHC antibodies.
Michael B. Brenner ; Christina M. Parker, Method of treating ulcerative colitis or crohn's disease by administering an antibody to.alpha.E.beta.7 integrin.
Leland John K. ; Shah Haresh P. ; Kenten John H. ; Goodman Jack E. ; Lowke George E. ; Namba Yuzaburo,JPX ; Blackburn Gary F. ; Massey Richard J., Methods and apparatus for improved luminescence assays.
Leland Jonathan K. ; Shah Haresh P. ; Kenten John H. ; Goodman Jack E. ; Lowke George E. ; Blackburn Gary F. ; Massey Richard J., Methods and apparatus for improved luminescence assays.
Verrant, John; Connelly, Mark Carle; Foulk, Brad; Kagan, Michael T; Swennenhuis, Joost F; Terstappen, Leon W. M. M.; Tibbe, Arjan G. J., Methods and composition to generate unique sequence DNA probes, labeling of DNA probes and the use of these probes.
Terstappen,Leon W. M. M.; Rao,Galla Chandra; Uhr,Jonathan W.; Racila,Emilian V.; Liberti,Paul A., Methods and reagents for the rapid and efficient isolation of circulating cancer cells.
Leland John K. ; Shah Haresh P. ; Kenten John H. ; Goodman Jack E. ; Lowke George E. ; Namba Yuzaburo,JPX ; Blackburn Gary F. ; Massey Richard J., Methods for improved particle electrochemiluminescence assays.
Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogeneous sample.
Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogeneous sample.
Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogeneous sample.
Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogenous sample.
Liberti Paul A. (Huntingdon Valley PA) Rao Galla C. (Princeton NJ) Chiarappa Joseph N. (Flemington NJ), Methods for the manufacture of magnetically responsive particles.
Barlow Eve H. ; Carroll ; III Eddie ; Connolly Joseph E. ; Lee Michael J. ; Martinelli Richard A. ; Unger John T., Non-separation specific binding chemiluminescent assay.
McMichael Andrew James (Horton-cum-Studley GB2) Nixon Douglas Fraser (Headington GB2) Townsend Alain Robert Michael (Oxford GB2), Peptide fragments of HIV.
Arnold ; Jr. Lyle J. (San Diego CA) Nelson Norman C. (San Diego CA) Reynolds Mark A. (La Jolla CA) Waldrop ; III Alexander A. (San Diego CA), Polycationic supports and nucleic acid purification separation and hybridization.
Van Alstine James M. (Montesano AL) Harris J. Milton (Huntsville AL) Shafer Steve (Huntsville AL) Snyder Robert S. (Huntsville AL) Herren Blair (Arab AL), Polymer-coated surfaces to control surface zeta potential.
Siiman Olavi (Davie FL) Burshteyn Alexander (Hialeah FL) Gupta Ravinder K. (Pembroke Pines FL), Polymeric particles having a biodegradable gelatin or aminodextran coating and process for making same.
Siiman Olavi ; Burshteyn Alexander ; Gupta Ravinder K., Polymeric particles having a biodegradable gelatin or aminodextran coating and processes for making same.
Chagnon Mark S. ; Ferris John R. ; Carter Michelle J. ; Hamilton Tracy J. ; Gray Maria A., Preparation of sub 100 A magnetic particles and magnetic molecular switches.
Hayashizaki, Yoshihide; Kimura, Yasumasa; Usui, Kengo; Tanaka, Yuki; Kawai, Yuki, Primer set for isothermal amplication of a target nucleic acid sequence.
Piasio,Roger N.; Turner,Nathan, Process for (A) separating biological/ligands from dilute solutions and (B) conducting an immunochromatographic assay thereof employing superparamagnetic particles throughout.
Piasio,Roger N.; Turner,Nathan, Process for (A) separating biological/ligands from dilute solutions and (B) conducting an immunochromatographic assay thereof employing superparamagnetic particles throughtout.
Ugelstad John (Trondheim NOX) Ellingsen Turid (Trondheim NOX) Berge Arvid (Trondheim NOX) Helgee Oskar B. (Mnlycke SEX), Process for preparing magnetic polymer particles.
Tsuda Shiro,JPX ; Heckman Kacey Wiley ; Hirota Yasutake,JPX ; Borduz Stefan ; Borduz Lucian, Process for producing a magnetic fluid and composition therefor.
Seider Robert J. (Ransomville NY) Guichelaar Philip J. (Williamsville NY) Anderson Robert O. (Akron NY), Production of silicon carbide with automatic separation of a high grade fraction.
Russell Inge,CAX ; Dowhanick Terrance M.,CAX ; Stewart Robert J.,CAX, Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer.
Gustafson Eric K. (Palo Alto CA) Allen Jimmy D. (Cupertino CA) Cobb Michael E. (San Jose CA), Solid-phase binding assay system for interferometrically measuring analytes bound to an active receptor.
Ni, Wei-Chao; Eustace, Daniel W.; Chang, Steve Chin-Shen, Stabilization of particles and reduction of sample discordance in immunoassays using casein coating of particles.
Wei-Chao Ni ; Daniel W. Eustace ; Steve Chin-Shen Chang, Stabilization of particles and reduction of sample discordance in immunoassays using casein coating of particles.
Arnquist, David C.; Barnes, III, Grady; Button, Richard D.; Dunn, Chadwick M.; East, Jr., Richard C.; Fritchie, Patrick P.; Galitz, Charles M.; Gardner, Gregory E.; Grandone, Cass J.; Gray, Robert C., Structure for determination of item of interest in a sample.
Verrant, John A; Tibbe, Arjan G. J.; Foulk, Brad; Swennenhuis, Joost F.; Terstappen, Leon W. M. M.; Connelly, Mark Carle; Kagan, Michael T., System for the detection and enumeration of suspect target cells in a mixed cell population.
Ueno, Shigehiro; Taguchi, Yosuke; Shimogawara, Masaya, Transition metal compound-containing nanoparticle and method for producing the same, ink for positive hole injection transport layer, device comprising positive hole injection transport layer and method for producing the same.
Ueno, Shigehiro; Taguchi, Yosuke; Shimogawara, Masaya, Transition metal compound-containing nanoparticle and method for producing the same, ink for positive hole injection transport layer, device comprising positive hole injection transport layer and method for producing the same.
Weisburg, William G.; Shaw, Jay H.; Becker, Michael M.; Majlessi, Mehrdad R.; Brentano, Steven T.; Nunomura, Kiyotada, Two-step hybridization and capture of a polynucleotide.
Cheon, Jin-Woo; Jun, Young-Wook; Choi, Jin-Sil, Water-soluble nanoparticles stabilized with multi-functional group ligands and method of preparation thereof.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.