Novel alpha-glycosylated soybean glycosides wherein one or more glucosyl residues are bound to the soybean glycoside moiety are prepared. Alpha-glycosylation can be enzymatically achieved by subjecting an aqueous solution containing a soybean glycoside, e.g. soyasaponin or isoflavonoid, and an alpha
Novel alpha-glycosylated soybean glycosides wherein one or more glucosyl residues are bound to the soybean glycoside moiety are prepared. Alpha-glycosylation can be enzymatically achieved by subjecting an aqueous solution containing a soybean glycoside, e.g. soyasaponin or isoflavonoid, and an alpha-glucosyl saccharide to an alpha-glucosyl transferase. Suitable alpha-glucosyl saccharides are liquefied starch, partially hydrolyzed starch and sucrose. Alpha-glucosidase, alpha-amylase, cyclodextrin glucanotransferase, dextransucrase, dextran dextrinase and amylosucrase are usable as the alpha-glucosyl transferase. Since in the alpha-glycosylated soybean glycosides the undesirable bitter, astringent, harsh and lingering choky tastes which are common in intact soybean glycosides are reduced or even eliminated by the alpha-glycosylation, they are suitable for use in food products or orally-usable products wherein taste is an important factor.
대표청구항▼
1. A process for producing alpha-glycosyl soybean glycoside or a mixture containing same, which process comprises: providing an aqueous solution containing a soybean glycoside and an alpha-glucosyl saccharide; and subjecting the solution to an alpha-glucosyl transferase. 2. A process as set fo
1. A process for producing alpha-glycosyl soybean glycoside or a mixture containing same, which process comprises: providing an aqueous solution containing a soybean glycoside and an alpha-glucosyl saccharide; and subjecting the solution to an alpha-glucosyl transferase. 2. A process as set forth in claim 1, wherein said alpha-glucosyl transferase is a member selected from the group consisting of alpha-glucosidase, alpha-amylase, cyclodextrin glucanotransferase, dextransucrase, dextran dextrinase and amylosucrase. 3. A process as set forth in claim 1, which process comprises: providing an aqueous solution containing a soybean glycoside and an alpha-glucosyl saccharide; subjecting the solution to an alpha-glucosyl transferase under the conditions sufficient to effect a substantial enzymatic transferring reaction; and recovering the resultant alpha-glycosyl soybean glycoside. 4. A process as set forth in claim 1, wherein said alpha-glucosyl saccharide is a member selected from the group consisting of gelatinized starch, liquefied starch, partial starch hydrolysate, maltose, maltotriose, maltotetraose, sucrose and mixture thereof. 5. A process as set forth in claim 4, wherein the DE of the gelatinized starch, liquefied starch or partial starch hydrolysate is up to 60. 6. A process as set forth in claim 1, which process comprises: providing an aqueous solution containing 0.01-30 w/w % soybean glycoside and 1-50 w/w % alpha-glucosyl saccharide; and subjecting the solution to an alpha-glucosyl transferase at a pH in the range of 3-10 and a temperature in the range of 20°-80° C. for a period sufficient to form a substantial amount of alpha-glycosyl soybean glycoside. 7. A process as set forth in claim 1, wherein the weight ratio of soybean glycoside to alpha-glucosyl saccharide is in the range of 0.5-500 on the basis of dry solid. 8. A process as set forth in claim 1, wherein said soybean glycoside is a member selected from the group consisting of soyasaponin, isoflavonoid and mixture thereof. 9. A process as set forth in claim 1, wherein a soybean milk is used as the source for soybean glycoside. 10. An orally-usable composition which contains an alpha-glycosyl soybean glycoside. 11. A composition as set forth in claim 10, which is in the form of paste. 12. A composition as set forth in claim 10, which is in the form of solid. 13. A composition as set forth in claim 10, wherein said alpha glycosyl soybean glycoside is prepared by providing an aqueous solution containing a soybean glycoside and an alpha-glucosyl saccharide; and subjecting the solution to an alpha-glucosyl transferase. 14. A composition as set forth in claim 10, which is a member selected from the group consisting of food, drink, pet food, feed, cosmetics, drug and tobacco. 15. A composition as set forth in claim 10, which additionally contains soybean glycoside and alpha-glucosyl saccharide. 16. A composition as set forth in claim 10, wherein said alpha-glycosyl soybean glycoside is a member selected from the group consisting of alpha-glycosyl soyasaponin, alpha-glycosyl isoflavonoid and mixture thereof. 17. The composition of claim 10 which is in the form of a food product. 18. Alpha-glycosyl soybean glycoside, which has a chemical structure wherein one or more alpha-glucosyl residues are bound to a soybean glycoside moiety in alpha-fashion. 19. Alpha-glycosyl soybean glycoside as set forth in claim 18, wherein said soybean glycoside is a member selected from the group consisting of soyasaponin, isoflavonoid and mixture thereof. 20. A process for producing food product, said process comprises: incorporating a substantial amount of alpha-glycosyl soybean glycoside into a food product. 21. A process as set forth in claim 20, wherein said food product is a food or drink. 22. A process as set forth in claim 20, wherein said food product is a member selected from the group consisting of pet food, feed, tobacco, cosmetic or drug. 23. A process as set forth in claim 20, wherein said food product is in the form of liquid. 24. A process as set forth in claim 20, wherein said food product is in the form of paste. 25. A process as set forth in claim 20, wherein said food product is in the form of solid. 26. A process as set forth in claim 20, wherein said alpha-glycosyl soybean glycoside is obtained by providing an aqueous solution containing a soybean glycoside and an alpha-glucosyl saccharide; and subjecting the solution to an alpha-glucosyl transferase. 27. A process as set forth in claim 20, wherein said alpha-glycosyl soybean glycoside is a member selected from the group consisting of alpha-glycosyl soyasaponin, alpha-glycosyl isoflavonoid and mixture thereof. 28. In an orally-usable composition wherein a soybean glycoside is incorporated, the improvement whereby the taste quality of the composition is extremely improved, comprising partially or completely replacing the soybean glycoside with its alpha-glycosyl derivative. 29. The improved orally-usable composition of claim 28, wherein said orally usable composition is a food product. 30. A method for purifying alpha-glycosyl soybean glycoside, said method containing the steps of: providing an aqueous solution of alpha-glycosyl soybean glycoside wherein impurities are present; allowing the solution to contact with a magnesia adsorbent thereby to adsorb the impurities thereon; and recovering the alpha-glycosyl soybean glycoside from the resultant non-adsorbed part. 31. A method as set forth in claim 30, wherein said alpha-glycosyl soybean glycoside is a member selected from the group consisting of alpha-glycosyl soyasaponin, alpha-glycosyl isoflavonoid and mixture thereof. 32. A method for recovering alpha-glycosyl soybean glycoside form its mixture with alpha-glucosyl saccharide, said method containing the steps of: providing an aqueous solution containing alpha-glycosyl soybean glycoside and alpha-glucosyl saccharide; allowing the solution to contact with a synthetic macroreticular resin thereby to adsorb the alpha-glycosyl soybean glycoside thereon; allowing the resin to contact with a low alcohol solution; and recovering the alpha-glycosyl soybean glycoside from the eluate. 33. A method as set forth in claim 32, wherein said alpha-glycosyl soybean glycoside is a member selected from the group consisting of alpha-glycosyl soyasaponin, alpha-glycosyl isoflavonoid and mixture thereof.
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