Protein product, process for making it and pharmaceutical composition containing said protein
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-039/36
A61K-039/00
A61K-045/02
A61K-037/00
출원번호
US-0478108
(1983-03-23)
우선권정보
FR-0005241 (1982-03-26)
발명자
/ 주소
David, Bernard
Peltre, Gabriel
Mecheri, Salah
출원인 / 주소
Institut Pasteur
대리인 / 주소
Oblon, Fisher, Spivak, McClelland & Maier
인용정보
피인용 횟수 :
15인용 특허 :
0
초록▼
New product consisting of glycoprotein having a molecular weight of about 14,000 and an isoelectric point of about 4.6, its absorption spectrum having an absorption of ε=0.12 units of optical density (O.D.) at 280 nm for an aqueous solution of 1 mg (dry weight) per ml of water. This product has immu
New product consisting of glycoprotein having a molecular weight of about 14,000 and an isoelectric point of about 4.6, its absorption spectrum having an absorption of ε=0.12 units of optical density (O.D.) at 280 nm for an aqueous solution of 1 mg (dry weight) per ml of water. This product has immunoregulating properties and is useful in allergic therapeutic composition.
대표청구항▼
1. A glycoprotein having a molecular weight of about 14,000 daltons and an isoelectric point of about 4.6; a 1 mg/ml aqueous solution of said glycoprotein having an absorption of ε=0.12 units of optical density at 280 nm; said glycoprotein being isolated by a process comprising: (a) admixing poll
1. A glycoprotein having a molecular weight of about 14,000 daltons and an isoelectric point of about 4.6; a 1 mg/ml aqueous solution of said glycoprotein having an absorption of ε=0.12 units of optical density at 280 nm; said glycoprotein being isolated by a process comprising: (a) admixing pollen of Dactylis glomerata with an aqueous medium, thereby extracting water-soluble products; (b) separating the aqueous extract from insoluble matter; (c) subjecting said aqueous extract to fractionation by molecular weight and isolectric point; and (d) isolating said glycoprotein. 2. A process for obtaining a glycoprotein comprising: (a) admixing pollen of Dactylis glomerata with an aqueous medium, thereby extracting water-soluble products; (b) separating the thus obtained aqueous extract from insoluble matter; (c) subjecting said aqueous extract to fractionation by molecular weight and isoelectric point; and (d) isolating the glycoprotein having a molecular weight of about 14,000 daltons and an isoelectric point of about 4.6. 3. A process according to claim 2, wherein the aqueous medium is distilled water. 4. A process according to claim 1, comprising operating at about room temperature. 5. A process according to claim 2, wherein step (c) is carried out by isoelectrofocusing. 6. A process according to claim 2, wherein step (c) is carried out by preparative chromatofocusing. 7. A process for obtaining a glycoprotein comprising (a) admixing pollen of orchard grass or Dactylis glomerata with an aqueous medium, thereby extracting water-soluble products; (b) separating the thus obtained aqueous extract from insoluble matter; (c) subjecting the extract to fractionation by molecular weight and isoelectric point; and (d) isolating the glycoprotein having a molecular weight of about 14,000 daltons and an isoelectric point of about 4.6. 8. A glycoprotein having a molecular weight of about 14,000 daltons and an isoelectric point of about 4.6; a 1 mg/ml aqueous solution of said glycoprotein having an absorption of ε=0.12 units of optical density at 280 nm; said glycoprotein being obtained by the process according to claim 7. 9. A method for inhibiting the synthesis of an immunoglobulin selected from the group consisting of homocytotropic IgE and IgG in a subject afflicted with increased production of said immunoglobulin, comprising administering to the subject an amount of a glycoprotein having a molecular weight of about 14,000 daltons, an isoelectric point of about 4.6, and an absorption of ε=0.12 units of optical density at 280 nm; effective to inhibit the synthesis of said immunoglobulins. 10. A pharmaceutical composition comprising: (a) an effective quantity of a glycoprotein having a molecular weight of about 14,000 daltons, an isoelectric point of about 4.6, and an absorption of ε=0.12 units of optical density at 280 nm, for the treatment of pollinosis; and (b) a pharmaceutically-acceptable vehicle adapted to oral or parenteral administration, or injection or inhalation. 11. The composition of claim 10, wherein the amount of the glycoprotein is between about 1 μg and 100 mg. 12. The composition of claim 10, in oral form, wherein the amount of the glycoprotein is up to about 10 mg. 13. The composition of claim 10 for topical application, wherein the glycoprotein is present in an amount of up to 100 mg. 14. The composition of claim 10, in injectable form, wherein the amount of the glycoprotein is between about 1 μg and 5 mg. 15. A pharmaceutical composition comprising: (a) an amount of a glycoprotein having a molecular weight of about 14,000 daltons, an isoelectric point of about 4.6 and an absorption of ε=0.12 units of optical density at 280 nm effective for inhibiting the production of an orchard grass anti-pollen immunoglobulin selected from the group consisting of IgE and IgG; and (b) a pharmaceutically-acceptable vehicle. 16. The composition of claim 10 in the form of an aerosol. 17. The composition of claim 10 in aqueous or oily form. 18. The composition of claim 17 in the form of a nasal, ophthalmic or inhalation preparation. 19. The composition of claim 12, in the form of gelules, capsules, or tablets. 20. A method of treating allergic reactions produced by the pollen of graminaceous plants, in a subject susceptible to said reactions, comprising administering to the subject an amount of a glycoprotein having a molecular weight of about 14,000 daltons, an isoelectric point of about 4.6, and an absorption of ε=0.12 units of optical density at 280 nm, effective for treating said allergic reactions.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허를 인용한 특허 (15)
Koganov, Michael, Bioactive botanical cosmetic compositions and processes for their production.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.