초록 ▼

An improved process for the reduction of the nitrate content of tobacco materials via dissimilatory denitrification is disclosed wherein more rapid reduction of nitrate to nitrogen gas is effected by utilizing a vacuum during incubation. The process comprises inoculating nitrate-containing tobacco m

An improved process for the reduction of the nitrate content of tobacco materials via dissimilatory denitrification is disclosed wherein more rapid reduction of nitrate to nitrogen gas is effected by utilizing a vacuum during incubation. The process comprises inoculating nitrate-containing tobacco material with a microorganism capable of dissimilatory denitrification and thereafter incubating the tobacco material under conditions whereby the nitrate is reduced to nitrogen gas via dissimilatory denitrification while applying a vacuum.

대표청구항 ▼

A process for dissimilatory denitrification of nitrate in tobacco material comprising: a. inoculating tobacco material with a sufficient amount of a microorganism capable of dissimilatory denitrification to provide an initial concentration of at least 1×107 cells per milliliter of said microorganism

A process for dissimilatory denitrification of nitrate in tobacco material comprising: a. inoculating tobacco material with a sufficient amount of a microorganism capable of dissimilatory denitrification to provide an initial concentration of at least 1×107 cells per milliliter of said microorganism; and b. incubating the inoculated tobacco material with agitation under conditions such that nitrate in the tobacco material is reduced to nitrogen gas via dissimilatory denitrification while maintaining a vaccum up to a maximum level of about 500 mm Hg.