초록 ▼

Methods and compositions are provided for performing an assay on whole blood samples. The method is for a determination of an analyte which is a member of a specific binding pair (sbp) consisting of ligand and homologous receptor. The method involves a binding agent for the red blood cells in such s

Methods and compositions are provided for performing an assay on whole blood samples. The method is for a determination of an analyte which is a member of a specific binding pair (sbp) consisting of ligand and homologous receptor. The method involves a binding agent for the red blood cells in such sample, a solid bibulous element to which is bound at least one sbp member, and a signal-producing system. The method comprises combining the whole blood sample, the binding agent, and none or, where appropriate, one or more members of the signal producing system. The medium is next contacted with a portion of a solid bibulous element to which is bound one of the members of the specific binding pair to allow the medium to traverse such element (immunochromatography). The solid bibulous element is contacted with any remaining members of the signal producing system. Signal resulting from the signal producing system is detected and is related to the amount of the analyte in the sample.

대표청구항 ▼

A method for detecting the presence of a member of a specific binding pair (“sbp member”) in a whole blood sample, said sbp member selected from the group consisting of ligand and homologous receptor, where said method involves (1) a binding agent for red blood cells in said sample; (2) a solid bibu

A method for detecting the presence of a member of a specific binding pair (“sbp member”) in a whole blood sample, said sbp member selected from the group consisting of ligand and homologous receptor, where said method involves (1) a binding agent for red blood cells in said sample; (2) a solid bibulous element to which is bound at least one sbp member; and (3) a signal producing system capable of producing a detectable signal, the members of which include at least one label which is bound to an sbp member, said method comprising: (a) combining in an aqueous medium a whole blood sample, a binding agent for aggregating red blood cells in said sample, and fewer than all the members of the signal producing system, (b) contacting a portion of said bibulous carrier with said aqueous medium to allow said medium to diffuse along said bibulous element and said aggregated red blood cells to concentrate in an area on said element adjacent the air/liquid interface, (c) contacting said bibulous element with any remaining members of the signal producing system, and (d) detecting the signal as a result of said signal producing system, wherein said signal is related to the amount of said sbp member in said sample.