초록 ▼

A process for exposing live cells to a soluble bone protein and transferring the cells into appropriate skeletal sites to initiate or enhance local chondrogenesis or osteogenesis. Isogenic fibroblasts are exposed in vitro to a soluble bone protein capable of stimulating in vitro and/or in vivo a cho

A process for exposing live cells to a soluble bone protein and transferring the cells into appropriate skeletal sites to initiate or enhance local chondrogenesis or osteogenesis. Isogenic fibroblasts are exposed in vitro to a soluble bone protein capable of stimulating in vitro and/or in vivo a chondrogenic response. The fibroblasts are then transferred in vivo in a manner effective to prevent diffusion away from the implantation site so that the desired chondro/osteogenesis reaction can proceed. Delivery systems include: combining the activated fibroblasts with a biodegradable matrix; suspending the activated fibroblasts in a solution suitable for intra-articular injection; and attaching the fibroblasts to allografts and prosthetic devices.

대표청구항 ▼

In a method of stimulating in vivo growth of skeletal tissue the improvement comprising the steps of combining soluble bone protein purified to a state effective to initiate chondrogenesis with fibroblast cells to form a protein-cell combination and placing the protein-cell combination in condition

In a method of stimulating in vivo growth of skeletal tissue the improvement comprising the steps of combining soluble bone protein purified to a state effective to initiate chondrogenesis with fibroblast cells to form a protein-cell combination and placing the protein-cell combination in condition for in vivo administration. In a method of stimulating in vivo growth of skeletal tissue the improvement comprising the steps of combining soluble bone protein purified to a state effective to initiate chondrogenesis with fibroblast cells to form a protein-cell combination, and further combining the protein-cell combination with a biodegradable carrier to form a composite mass. A method of stimulating in vivo growth of skeletal tissue at a site of a joint cavity articular comprising the steps of: contacting fibroblast cells with soluble bone protein purified to a state effective to initiate chondrogenesis; combining the cells with an injectable solution; and injecting the cells intra-articularly.